anti-PI3 Kinase p85 / p55 phospho (Tyr467 / Tyr199) antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG51810
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PI3 Kinase p85 / p55 phospho (Tyr467 / Tyr199) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 特异性 | The antibody reacts PI3 Kinase p85 and p55 only when phosphorylated at tyrosine 467 of p85 and tyrosine 199 of p55. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PI3 Kinase p85 / p55 |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around phosphorylation site of Tyrosine 467 (L-Y(p)-E-E-Y) derived from Human PI3 Kinase p85 / p55. |
| 偶联标记 | Un-conjugated |
| 別名 | GRB1; PI3-kinase subunit p85-alpha; Phosphatidylinositol 3-kinase regulatory subunit alpha; IMD36; PtdIns-3-kinase regulatory subunit alpha; p85-ALPHA; p85; AGM7; PtdIns-3-kinase regulatory subunit p85-alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha |
应用说明
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PIK3R1 |
| 全名 | phosphoinositide-3-kinase, regulatory subunit 1 (alpha) |
| 背景介绍 | Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011] |
| 生物功能 | Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress-and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923). [UniProt] |
| 产品亮点 | Related products: PI3K P85 antibodies; Anti-Rabbit IgG secondary antibodies; |
| 研究领域 | Cancer antibody; Immune System antibody; Signaling Transduction antibody |
| 预测分子量 | 84 kDa |
| 翻译后修饰 | Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation. Phosphorylated. Tyrosine phosphorylated in response to signaling by FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylated by CSF1R. Phosphorylated by ERBB4. Phosphorylated on tyrosine residues by TEK/TIE2. Dephosphorylated by PTPRJ. Phosphorylated by PIK3CA at Ser-608; phosphorylation is stimulated by insulin and PDGF. The relevance of phosphorylation by PIK3CA is however unclear (By similarity). Phosphorylated in response to KIT and KITLG/SCF. Phosphorylated by FGR. |
检测图片 (1)
ARG51810 anti-PI3 Kinase p85 / p55 phospho (Tyr467 / Tyr199) antibody WB image
Western blot: Extracts from 293 cells untreated or treated with sorbitol stained with ARG51810 anti-PI3 Kinase p85 / p55 phospho (Tyr467 / Tyr199) antibody.
参考文献
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