anti-PIK3CG / p110 gamma antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
CAT.NO. : ARG43223
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PIK3CG / p110 gamma |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PIK3CG / p110 gamma |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-200 of Human PIK3CG / p110 gamma (NP_002640.2). |
| 偶联标记 | Un-conjugated |
| 別名 | PI3CG; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma; PI3K; Phosphoinositide-3-kinase catalytic gamma polypeptide; PtdIns-3-kinase subunit p110-gamma; EC 2.7.1.153; PtdIns-3-kinase subunit gamma; p120-PI3K; EC 2.7.11.1; p110gamma; PI3-kinase subunit gamma; PI3Kgamma; PI3K-gamma; PIK3; Serine/threonine protein kinase PIK3CG; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Jurkat | ||||||||
| 实际分子量 | ~ 125 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P48736 Human Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma iso Swiss-port # Q9JHG7 Mouse Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma iso |
|---|---|
| 基因名称 | PIK3CG |
| 全名 | phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit gamma |
| 背景介绍 | Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I catalytic subunit of PI3K. Like other class I catalytic subunits (p110-alpha p110-beta, and p110-delta), the encoded protein binds a p85 regulatory subunit to form PI3K. This gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jun 2015] |
| 生物功能 | Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Links G-protein coupled receptor activation to PIP3 production. Involved in immune, inflammatory and allergic responses. Modulates leukocyte chemotaxis to inflammatory sites and in response to chemoattractant agents. May control leukocyte polarization and migration by regulating the spatial accumulation of PIP3 and by regulating the organization of F-actin formation and integrin-based adhesion at the leading edge. Controls motility of dendritic cells. Together with PIK3CD is involved in natural killer (NK) cell development and migration towards the sites of inflammation. Participates in T-lymphocyte migration. Regulates T-lymphocyte proliferation and cytokine production. Together with PIK3CD participates in T-lymphocyte development. Required for B-lymphocyte development and signaling. Together with PIK3CD participates in neutrophil respiratory burst. Together with PIK3CD is involved in neutrophil chemotaxis and extravasation. Together with PIK3CB promotes platelet aggregation and thrombosis. Regulates alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) adhesive function in platelets downstream of P2Y12 through a lipid kinase activity-independent mechanism. May have also a lipid kinase activity-dependent function in platelet aggregation. Involved in endothelial progenitor cell migration. Negative regulator of cardiac contractility. Modulates cardiac contractility by anchoring protein kinase A (PKA) and PDE3B activation, reducing cAMP levels. Regulates cardiac contractility also by promoting beta-adrenergic receptor internalization by binding to GRK2 and by non-muscle tropomyosin phosphorylation. Also has serine/threonine protein kinase activity: both lipid and protein kinase activities are required for beta-adrenergic receptor endocytosis. May also have a scaffolding role in modulating cardiac contractility. Contributes to cardiac hypertrophy under pathological stress. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis. [UniProt] |
| 细胞定位 | Cytoplasm. Cell membrane. [UniProt] |
| 预测分子量 | 126 kDa |
检测图片 (3)
ARG43223 anti-PIK3CG / p110 gamma antibody ICC/IF image
Immunofluorescence: NIH/3T3 cells stained with ARG43223 anti-PIK3CG / p110 gamma antibody at 1:100 dilution.
ARG43223 anti-PIK3CG / p110 gamma antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse spleen tissue stained with ARG43223 anti-PIK3CG / p110 gamma antibody at 1:100 dilution.
ARG43223 anti-PIK3CG / p110 gamma antibody WB image
Western blot: 25 µg of Jurkat cell lysate stained with ARG43223 anti-PIK3CG / p110 gamma antibody at 1:1000 dilution.
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