anti-PLAP / Placental alkaline phosphatase antibody
产品描述 | Rabbit Polyclonal antibody recognizes PLAP / Placental alkaline phosphatase |
---|---|
反应物种 | Hu |
应用 | FACS, ICC/IF, IHC-P, WB |
宿主 | Rabbit |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | PLAP / Placental alkaline phosphatase |
抗原物种 | Human |
抗原 | Synthetic peptide corresponding to a.a. 403-433 of Human PLAP / Placental alkaline phosphatase. |
偶联标记 | Un-conjugated |
Protein Full name | Alkaline phosphatase, placental type |
別名 | PALP; PLAP; EC 3.1.3.1; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1; Alkaline phosphatase, placental type; ALP |
应用建议 |
| ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
实际分子量 | ~ 70 kDa |
形式 | Liquid |
---|---|
纯化 | Affinity purification with immunogen. |
缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
抗菌剂 | 0.05% Sodium azide |
稳定剂 | 4% Trehalose |
浓度 | 0.5 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
数据库连接 | Swiss-port # P05187 Human Alkaline phosphatase, placental type |
---|---|
基因名称 | ALPP |
全名 | alkaline phosphatase, placental |
背景介绍 | The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid Polyesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Jan 2015] |
生物功能 | Alkaline phosphatase that can hydrolyze various phosphate compounds. [UniProt] |
细胞定位 | Cell membrane; Lipid-anchor, GPI-anchor. [UniProt] |
预测分子量 | 58 kDa |
翻译后修饰 | Disulfide bond; Glycoprotein; GPI-anchor; Lipoprotein |
ARG43614 anti-PLAP / Placental alkaline phosphatase antibody IHC-P image
Immunohistochemistry: Human placenta ARG43614 anti-PLAP / Placental alkaline phosphatase antibody at 1:250 dilution.
ARG43614 anti-PLAP / Placental alkaline phosphatase antibody ICC/IF image
Immunofluorescence: HeLa cell stained with ARG43614 anti-PLAP / Placental alkaline phosphatase antibody at 1:250 dilution.
ARG43614 anti-PLAP / Placental alkaline phosphatase antibody WB image
Western blot: Human placenta cell lysates stained with ARG43614 anti-PLAP / Placental alkaline phosphatase antibody at 1:1000 dilution.
ARG43614 anti-PLAP / Placental alkaline phosphatase antibody FACS image
Flow Cytometry: SiHa cells stained with ARG43614 anti-PLAP / Placental alkaline phosphatase antibody at 1:500 / 1X10^6 cells.
ARG43614 anti-PLAP / Placental alkaline phosphatase antibody ICC/IF image
Immunofluorescence:Human placenta stained with ARG43614 anti-PLAP / Placental alkaline phosphatase antibody at 1:250 dilution.