anti-POLH / DNA Polymerase Eta antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes POLH / DNA Polymerase Eta
  • 反应物种: Hu, Ms, Rat
  • 应用: ICC/IF, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: POLH / DNA Polymerase Eta
  • 抗原物种: Human
  • 抗原: Recombinant fusion protein corresponding to aa. 20-320 of Human POLH (NP_006493.1).
  • Brand:
CAT.NO. : ARG58953
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes POLH / DNA Polymerase Eta
反应物种Hu, Ms, Rat
应用ICC/IF, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称POLH / DNA Polymerase Eta
抗原物种Human
抗原Recombinant fusion protein corresponding to aa. 20-320 of Human POLH (NP_006493.1).
偶联标记Un-conjugated
別名Xeroderma pigmentosum variant type protein; RAD30; RAD30A; XP-V; DNA polymerase eta; EC 2.7.7.7; RAD30 homolog A; XPV
应用说明
应用建议
应用推荐稀释比
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照HepG2
实际分子量70 kDa
属性
形式Liquid
纯化Affinity purified.
缓冲液PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂0.02% Sodium azide
稳定剂50% Glycerol
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 5429 Human POLH

GeneID: 80905 Mouse POLH

Swiss-port # Q9JJN0 Mouse DNA polymerase eta

Swiss-port # Q9Y253 Human DNA polymerase eta

基因名称POLH
全名polymerase (DNA directed), eta
背景介绍This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]
生物功能DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI to replication foci. [UniProt]
细胞定位Nucleus. [UniProt]
预测分子量78 kDa
翻译后修饰Monoubiquitinated by RCHY1/PIRH2; ubiquitination inhibits the ability of PolH to interact with PCNA and to bypass UV-induced lesions. [UniProt]
检测图片 (2)
  • ARG58953 anti-POLH / DNA Polymerase Eta antibody ICC/IF image

    Immunofluorescence: U2OS cells stained with ARG58953 anti-POLH / DNA Polymerase Eta antibody.

  • ARG58953 anti-POLH / DNA Polymerase Eta antibody WB image

    Western blot: 25 µg of HepG2 cell lysate stained with ARG58953 anti-POLH / DNA Polymerase Eta antibody at 1:1000 dilution.

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