anti-Progesterone Receptor antibody [SP42]

Key features and details

产品描述: Rabbit Monoclonal antibody [SP42] recognizes Progesterone Receptor
反应物种: Hu
应用: IHC-P
宿主: Rabbit
克隆: Monoclonal
克隆号: SP42
同位型: IgG
靶点名称: Progesterone Receptor
抗原物种: Human

Brand:

CAT.NO. : ARG53495

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Size:

100 μl

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Product Details

概述

产品描述	Rabbit Monoclonal antibody [SP42] recognizes Progesterone Receptor
反应物种	Hu
应用	IHC-P
宿主	Rabbit
克隆	Monoclonal
克隆号	SP42
同位型	IgG
靶点名称	Progesterone Receptor
抗原物种	Human
抗原	Recombinant protein encoding aa 412-526 of human progesterone receptor.
偶联标记	Un-conjugated
別名	PR; NR3C3; Nuclear receptor subfamily 3 group C member 3; Progesterone receptor

应用说明

应用建议

应用	推荐稀释比
IHC-P	1:400

应用说明

IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 30 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

阳性对照

Breast Carcinoma

属性

形式	Liquid
缓冲液	Tissue culture supernatant in TBS (pH 7.5), 1% BSA and
抗菌剂
稳定剂	1% BSA
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 5241 Human PGR Swiss-port # P06401 Human Progesterone receptor
基因名称	PGR
全名	progesterone receptor
背景介绍	This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. [provided by RefSeq, Jan 2011]
生物功能	The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation. Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation. Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone. [UniProt]
细胞定位	Nucleus
研究领域	Cancer antibody; Gene Regulation antibody; Neuroscience antibody; Signaling Transduction antibody
预测分子量	99 kDa
翻译后修饰	Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1. Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294. Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294. Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.

检测图片 (13)

ARG53495 anti-Progesterone Receptor antibody [SP42] IHC-P image
Immunohistochemistry: Human Bladder stained with ARG53495 anti-Progesterone Receptor antibody [SP42].
ARG53495 anti-Progesterone Receptor antibody [SP42] IHC-P image
Immunohistochemistry: Human Breast Carcinoma stained with ARG53495 anti-Progesterone Receptor antibody [SP42].
ARG53495 anti-Progesterone Receptor antibody [SP42] IHC-P image
Immunohistochemistry: Human Breast stained with ARG53495 anti-Progesterone Receptor antibody [SP42].
ARG53495 anti-Progesterone Receptor antibody [SP42] IHC-P image
Immunohistochemistry: Human Cervical SCC stained with ARG53495 anti-Progesterone Receptor antibody [SP42].
ARG53495 anti-Progesterone Receptor antibody [SP42] IHC-P image
Immunohistochemistry: Human Cervix stained with ARG53495 anti-Progesterone Receptor antibody [SP42].
ARG53495 anti-Progesterone Receptor antibody [SP42] IHC-P image
Immunohistochemistry: Human Endometrium stained with ARG53495 anti-Progesterone Receptor antibody [SP42].
ARG53495 anti-Progesterone Receptor antibody [SP42] IHC-P image
Immunohistochemistry: Human Fallopian Tube stained with ARG53495 anti-Progesterone Receptor antibody [SP42].
ARG53495 anti-Progesterone Receptor antibody [SP42] IHC-P image
Immunohistochemistry: Human Ovarian Adenocarcinoma stained with ARG53495 anti-Progesterone Receptor antibody [SP42].
ARG53495 anti-Progesterone Receptor antibody [SP42] IHC-P image
Immunohistochemistry: Human Ovary stained with ARG53495 anti-Progesterone Receptor antibody [SP42].
ARG53495 anti-Progesterone Receptor antibody [SP42] IHC-P image
Immunohistochemistry: Human Pancreas stained with ARG53495 anti-Progesterone Receptor antibody [SP42].
ARG53495 anti-Progesterone Receptor antibody [SP42] IHC-P image
Immunohistochemistry: Human Parathyroid Adenoma stained with ARG53495 anti-Progesterone Receptor antibody [SP42].
ARG53495 anti-Progesterone Receptor antibody [SP42] IHC-P image
Immunohistochemistry: Human Prostate stained with ARG53495 anti-Progesterone Receptor antibody [SP42].
ARG53495 anti-Progesterone Receptor antibody [SP42] IHC-P image
Immunohistochemistry: Human Uterus stained with ARG53495 anti-Progesterone Receptor antibody [SP42].

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