anti-Prothrombin antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes Prothrombin
  • 反应物种: Hu
  • 应用: FACS, IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: Prothrombin
  • 抗原物种: Human
  • 抗原: Recombinant protein corresponding to Y97-R124 of Human Prothrombin.
  • Brand:
CAT.NO. : ARG58569
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes Prothrombin
反应物种Hu
应用FACS, IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称Prothrombin
抗原物种Human
抗原Recombinant protein corresponding to Y97-R124 of Human Prothrombin.
偶联标记Un-conjugated
別名PT; EC 3.4.21.5; Prothrombin; THPH1; Coagulation factor II; RPRGL2
应用说明
应用建议
应用推荐稀释比
FACS1:150 - 1:500
IHC-P0.5 - 1 µg/ml
WB0.1 - 0.5 µg/ml
应用说明IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified.
缓冲液0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
抗菌剂0.05% Sodium azide
稳定剂4% Trehalose
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 2147 Human F2

Swiss-port # P00734 Human Prothrombin

基因名称F2
全名coagulation factor II (thrombin)
背景介绍Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 lead to various forms of thrombosis and dysprothrombinemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]
生物功能Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. [UniProt]
细胞定位Secreted, extracellular space. [UniProt]
预测分子量70 kDa
翻译后修饰The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.

N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). [UniProt]
检测图片 (4)
  • ARG58569 anti-Prothrombin antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human colon cancer tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with 1 µg/ml ARG58569 anti-Prothrombin antibody overnight at 4°C.

  • ARG58569 anti-Prothrombin antibody WB image

    Western blot: 50 µg of Human plasma lysate stained with ARG58569 anti-Prothrombin antibody at 0.5 µg/ml, overnight at 4°C, under reducing conditions.

  • ARG58569 anti-Prothrombin antibody FACS image

    Flow Cytometry: Human PBMC cells were blocked with 10% normal goat serum and then stained with ARG58569 anti-Prothrombin antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.

  • ARG58569 anti-Prothrombin antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with 1 µg/ml ARG58569 anti-Prothrombin antibody overnight at 4°C.

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