anti-PYCR1 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes PYCR1
  • 反应物种: Hu
  • 应用: FACS, IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: PYCR1
  • 抗原物种: Human
  • 抗原: KLH-conjugated synthetic peptide between aa. 291-319 of Human PYCR1.
  • Brand:
CAT.NO. : ARG42482
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes PYCR1
反应物种Hu
应用FACS, IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称PYCR1
抗原物种Human
抗原KLH-conjugated synthetic peptide between aa. 291-319 of Human PYCR1.
偶联标记Un-conjugated
別名PIG45; EC 1.5.1.2; P5CR 1; Pyrroline-5-carboxylate reductase 1, mitochondrial; PRO3; P5C reductase 1; P5C; ARCL3B; P5CR; ARCL2B; PP222; PYCR
应用说明
应用建议
应用推荐稀释比
FACS1:10 - 1:50
IHC-P1:50 - 1:100
WB1:1000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照WiDr
实际分子量~ 33 kDa
属性
形式Liquid
纯化Purification with Protein A and immunogen peptide.
缓冲液PBS and 0.09% (W/V) Sodium azide.
抗菌剂0.09% (W/V) Sodium azide.
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 5831 Human PYCR1

Swiss-port # P32322 Human Pyrroline-5-carboxylate reductase 1, mitochondrial

基因名称PYCR1
全名pyrroline-5-carboxylate reductase 1
背景介绍This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
生物功能Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress. [UniProt]
细胞定位Mitochondrion. [UniProt]
预测分子量33 kDa
检测图片 (3)
  • ARG42482 anti-PYCR1 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human colon carcinoma tissue stained with ARG42482 anti-PYCR1 antibody.

  • ARG42482 anti-PYCR1 antibody WB image

    Western blot: 35 µg of WiDr cell lysate stained with ARG42482 anti-PYCR1 antibody.

  • ARG42482 anti-PYCR1 antibody FACS image

    Flow Cytometry: HepG2 cells stained with ARG42482 anti-PYCR1 antibody (right histogram) or without primary antibody as control (left histogram), followed by incubation with FITC labelled secondary antibody.

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