anti-RBBP4 / RbAp48 antibody [9F3]

Key features and details

产品描述: Mouse Monoclonal antibody [9F3] recognizes RBBP4 / RbAp48
反应物种: Hu, Ms, Rat
应用: FACS, IHC-P, WB
宿主: Mouse
克隆: Monoclonal
克隆号: 9F3
同位型: IgG1
靶点名称: RBBP4 / RbAp48
抗原物种: Human

Brand:

CAT.NO. : ARG59051

US＄ Please choose

Size:

50 μg

Trail, Bulk size or Custom requests Please contact us

Product Details

概述

产品描述	Mouse Monoclonal antibody [9F3] recognizes RBBP4 / RbAp48
反应物种	Hu, Ms, Rat
应用	FACS, IHC-P, WB
宿主	Mouse
克隆	Monoclonal
克隆号	9F3
同位型	IgG1
靶点名称	RBBP4 / RbAp48
抗原物种	Human
抗原	Synthetic peptide corresponding to aa. 395-425 of Human RbAp48 (EDNIMQVWQMAENIYNDEDPEGSVDPEGQGS)
偶联标记	Un-conjugated
別名	Retinoblastoma-binding protein 4; Retinoblastoma-binding protein p48; Chromatin assembly factor I p48 subunit; Nucleosome-remodeling factor subunit RBAP48; RBBP-4; NURF55; lin-53; CAF-I p48; CAF-I 48 kDa subunit; Chromatin assembly factor 1 subunit C; CAF-1 subunit C; RBAP48; Histone-binding protein RBBP4

应用说明

应用建议

应用	推荐稀释比
FACS	1:150 - 1:500
IHC-P	0.5 - 1 µg/ml
WB	0.1 - 0.5 µg/ml

应用说明

IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

实际分子量

53 kDa

属性

形式	Liquid
纯化	Affinity purification with immunogen.
缓冲液	0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose.
抗菌剂	0.05% Sodium azide
稳定剂	4% Trehalose
浓度	0.5 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 19646 Mouse RBBP4 GeneID: 5928 Human RBBP4 Swiss-port # Q09028 Human Histone-binding protein RBBP4 Swiss-port # Q60972 Mouse Histone-binding protein RBBP4
基因名称	RBBP4
全名	retinoblastoma binding protein 4
背景介绍	This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
生物功能	Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex. [UniProt]
细胞定位	Nucleus. [UniProt]
预测分子量	48 kDa

检测图片 (10)

ARG59051 anti-RBBP4 / RbAp48 antibody [9F3] IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59051 anti-RBBP4 / RbAp48 antibody [9F3] at 2 µg/ml dilution, overnight at 4°C.
ARG59051 anti-RBBP4 / RbAp48 antibody [9F3] IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59051 anti-RBBP4 / RbAp48 antibody [9F3] at 1 µg/ml dilution, overnight at 4°C.
ARG59051 anti-RBBP4 / RbAp48 antibody [9F3] WB image
Western blot: 50 µg of samples under reducing conditions. A549, Jurkat, HeLa and PANC-1 cell lysates stained with ARG59051 anti-RBBP4 / RbAp48 antibody [9F3] at 0.5 µg/ml, overnight at 4°C.
ARG59051 anti-RBBP4 / RbAp48 antibody [9F3] WB image
Western blot: 50 µg of sample under reducing conditions. Jurkat, Rat thymus and Mouse spleen lysates stained with ARG59051 anti-RBBP4 / RbAp48 antibody [9F3] at 0.5 µg/ml dilution, overnight at 4°C.
ARG59051 anti-RBBP4 / RbAp48 antibody [9F3] FACS image
Flow Cytometry: SiHa cells were blocked with 10% normal goat serum and then stained with ARG59051 anti-RBBP4 / RbAp48 antibody [9F3] (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
ARG59051 anti-RBBP4 / RbAp48 antibody [9F3] IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59051 anti-RBBP4 / RbAp48 antibody [9F3] at 2 µg/ml dilution, overnight at 4°C.
ARG59051 anti-RBBP4 / RbAp48 antibody [9F3] IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59051 anti-RBBP4 / RbAp48 antibody [9F3] at 1 µg/ml dilution, overnight at 4°C.
ARG59051 anti-RBBP4 / RbAp48 antibody [9F3] IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59051 anti-RBBP4 / RbAp48 antibody [9F3] at 1 µg/ml dilution, overnight at 4°C.
ARG59051 anti-RBBP4 / RbAp48 antibody [9F3] IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse brain tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59051 anti-RBBP4 / RbAp48 antibody [9F3] at 1 µg/ml dilution, overnight at 4°C.
ARG59051 anti-RBBP4 / RbAp48 antibody [9F3] IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59051 anti-RBBP4 / RbAp48 antibody [9F3] at 1 µg/ml dilution, overnight at 4°C.

New Products

Mouse PD-1 ELISA kit

Human IL-12B p40 ELISA Kit

Human CD200R1 ELISA kit

Human IFN-α ELISA Kit

Human FGF basic/FGF2/bFGF ELISA Kit

Human CCL2/MCP-1 ELISA Kit