anti-RBP1 antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes RBP1
  • 反应物种: Hu, Ms
  • 预测物种: Cow
  • 应用: FACS, ICC/IF, IHC-P, WB
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: RBP1
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG63745
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes RBP1
反应物种Hu, Ms
预测物种Cow
应用FACS, ICC/IF, IHC-P, WB
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称RBP1
抗原物种Human
抗原VEGVVCKQVFKKVQ
偶联标记Un-conjugated
別名RBPC; CRBP-I; Cellular retinol-binding protein I; CRBP; CRBP1; Cellular retinol-binding protein; CRABP-I; Retinol-binding protein 1; CRBPI
应用说明
应用建议
应用推荐稀释比
FACS10 µg/ml
ICC/IF10 µg/ml
IHC-PAssay - dependent
WB0.5 - 3 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 19659 Mouse RBP1

GeneID: 5947 Human RBP1

Swiss-port # P09455 Human Retinol-binding protein 1

Swiss-port # Q00915 Mouse Retinol-binding protein 1

背景介绍This gene encodes the carrier protein involved in the transport of retinol (vitamin A alcohol) from the liver storage site to peripheral tissue. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
研究领域Gene Regulation antibody; Signaling Transduction antibody
预测分子量16 kDa
检测图片 (5)
  • ARG63745 anti-RBP1 antibody ICC/IF image

    Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG63745 anti-RBP1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.

  • ARG63745 anti-RBP1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63745 anti-RBP1 antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG63745 anti-RBP1 antibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Pancreas. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63745 anti-RBP1 antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG63745 anti-RBP1 antibody WB image

    Western blot: 35 µg of NIH/3T3 (A) and U251 (B) cell lysates (in RIPA buffer) stained with ARG63745 anti-RBP1 antibody at 2 µg/ml (A) and 0.5 µg/ml (B) dilutions and incubated at RT for 1 hour.

  • ARG63745 anti-RBP1 antibody FACS image

    Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG63745 anti-RBP1 antibody (blue line) at 10 µg/ml dilution and overnight, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).

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