anti-RBP1 antibody
产品描述 | Goat Polyclonal antibody recognizes RBP1 |
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反应物种 | Hu, Ms |
预测物种 | Cow |
应用 | FACS, ICC/IF, IHC-P, WB |
宿主 | Goat |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | RBP1 |
抗原物种 | Human |
抗原 | VEGVVCKQVFKKVQ |
偶联标记 | Un-conjugated |
別名 | RBPC; CRBP-I; Cellular retinol-binding protein I; CRBP; CRBP1; Cellular retinol-binding protein; CRABP-I; Retinol-binding protein 1; CRBPI |
应用建议 |
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应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
形式 | Liquid |
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纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
抗菌剂 | 0.02% Sodium azide |
稳定剂 | 0.5% BSA |
浓度 | 0.5 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
数据库连接 | |
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背景介绍 | This gene encodes the carrier protein involved in the transport of retinol (vitamin A alcohol) from the liver storage site to peripheral tissue. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] |
研究领域 | Gene Regulation antibody; Signaling Transduction antibody |
预测分子量 | 16 kDa |
ARG63745 anti-RBP1 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG63745 anti-RBP1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
ARG63745 anti-RBP1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63745 anti-RBP1 antibody at 5 µg/ml dilution followed by AP-staining.
ARG63745 anti-RBP1 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Pancreas. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63745 anti-RBP1 antibody at 5 µg/ml dilution followed by AP-staining.
ARG63745 anti-RBP1 antibody WB image
Western blot: 35 µg of NIH/3T3 (A) and U251 (B) cell lysates (in RIPA buffer) stained with ARG63745 anti-RBP1 antibody at 2 µg/ml (A) and 0.5 µg/ml (B) dilutions and incubated at RT for 1 hour.
ARG63745 anti-RBP1 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG63745 anti-RBP1 antibody (blue line) at 10 µg/ml dilution and overnight, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).