anti-RPL8 antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes RPL8
  • 反应物种: Hu
  • 预测物种: Ms, Rat, Dog
  • 应用: IHC-P, WB
  • 特异性: The reported variants represent identical protein (NP_000964.1 and NP_150644.1).
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: RPL8
  • Brand:
CAT.NO. : ARG64271
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes RPL8
反应物种Hu
预测物种Ms, Rat, Dog
应用IHC-P, WB
特异性The reported variants represent identical protein (NP_000964.1 and NP_150644.1).
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称RPL8
抗原物种Human
抗原KAGRAYHKYKAKRNC
偶联标记Un-conjugated
別名60S ribosomal protein L8; L8
应用说明
应用建议
应用推荐稀释比
IHC-P2.5 µg/ml
WB0.01 - 0.03 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 6132 Human RPL8

Swiss-port # P62917 Human 60S ribosomal protein L8

背景介绍Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
研究领域Gene Regulation antibody
预测分子量28 kDa
翻译后修饰Hydroxylated on His-216 by RIOX1. The modification is impaired by hypoxia.
检测图片 (3)
  • ARG64271 anti-RPL8 antibody WB image

    Western blot: HepG2 cell lysate (35 µg protein in RIPA buffer) stained with ARG64271 anti-RPL8 antibody at 0.01 µg/ml dilution.

  • ARG64271 anti-RPL8 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human spleen tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64271 anti-RPL8 antibody at 2.5 µg/ml dilution followed by AP-staining.

  • ARG64271 anti-RPL8 antibody IHC-P image

    Immunohistochemistry: Paraffin embedded Human Cerebral Cortex. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64271 anti-RPL8 antibody at 2.5 µg/ml dilution followed by AP-staining.

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