anti-STAT1 phospho (Tyr701) antibody [SM1351]
![anti-STAT1 phospho (Tyr701) antibody [SM1351]](/upload/image/products/ARG23741_WB_1_210_205.jpg)
![anti-STAT1 phospho (Tyr701) antibody [SM1351]](/upload/image/products/ARG23741_WB_1.jpg)
Key features and details
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- 预测物种:
- 应用:
- 特异性:
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- 同位型:
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CAT.NO. : ARG23741
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [SM1351] recognizes STAT1 phospho (Tyr701) |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat |
| 应用 | WB |
| 特异性 | The antibody detects 84 and 91 kDa stat1 variants on SDS-PAGE immunoblots of human A431 treated with EGF, as well as Jurkat and A431 cells treated with pervanadate. The antibody does not detect these variants in control cells. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | SM1351 |
| 同位型 | IgG1 |
| 靶点名称 | STAT1 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated phospho-specific peptide around Tyr701 of Human STAT1. This peptide sequence has high homology to the conserved tyrosine site in Rat and Mouse STAT1. |
| 偶联标记 | Un-conjugated |
| 別名 | ISGF-3; Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84; CANDF7; IMD31A; IMD31B; IMD31C; STAT91 |
应用说明
| 应用建议 |
| ||||
|---|---|---|---|---|---|
| 应用说明 | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 50% Glycerol and 1 mg/ml BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P42224 Human Signal transducer and activator of transcription 1-alpha/beta |
|---|---|
| 基因名称 | STAT1 |
| 全名 | signal transducer and activator of transcription 1, 91kDa |
| 背景介绍 | The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008] |
| 生物功能 | Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. [UniProt] |
| 产品亮点 | Related products: STAT1 antibodies; STAT1 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Exploring Antiviral Immune Response circNDUFB2, a circular RNA (circRNA), activates anti-tumor immunity |
| 预测分子量 | 87 kDa |
| 翻译后修饰 | Phosphorylated on tyrosine and serine residues in response to a variety of cytokines/growth hormones including IFN-alpha, IFN-gamma, PDGF and EGF. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Upon EGF stimulation, phosphorylation on Tyr-701 (lacking in beta form) by JAK1, JAK2 or TYK2 promotes dimerization and subsequent translocation to the nucleus. Growth hormone (GH) activates STAT1 signaling only via JAK2. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylation on Ser-727 by several kinases including MAPK14, ERK1/2 and CAMKII on IFN-gamma stimulation, regulates STAT1 transcriptional activity. Phosphorylation on Ser-727 promotes sumoylation though increasing interaction with PIAS. Phosphorylation on Ser-727 by PRKCD induces apoptosis in response to DNA-damaging agents. Phosphorylated on tyrosine residues when PTK2/FAK1 is activated; most likely this is catalyzed by a SRC family kinase. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates interferon-mediated signaling. Upon viral infection or IFN induction, phosphorylation on Ser-708 occurs much later than phosphorylation on Tyr-701 and is required for the binding of ISGF3 on the ISREs of a subset of IFN-stimulated genes IKBKE-dependent. Phosphorylation at Tyr-701 and Ser-708 are mutually exclusive, phosphorylation at Ser-708 requires previous dephosphorylation of Tyr-701. Sumoylated with SUMO1, SUMO2 and SUMO3. Sumoylation is enhanced by IFN-gamma-induced phosphorylation on Ser-727, and by interaction with PIAS proteins. Enhances the transactivation activity. ISGylated. [UniProt] |
检测图片 (1)
ARG23741 anti-STAT1 phospho (Tyr701) antibody [SM1351] WB image
Western blot: A431 cells untreated (left) or treated with 100 nM EGF for 60 min (right). The blots were stained with ARG23741 anti-STAT1 phospho (Tyr701) antibody [SM1351].
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