anti-STAT5A phospho (Tyr694) antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
CAT.NO. : ARG51552
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes STAT5A phospho (Tyr694) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | STAT5A |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around phosphorylation site of tyrosine 694 (D-G-Y(p)-V-K) derived from Human STAT5A. |
| 偶联标记 | Un-conjugated |
| 別名 | Signal transducer and activator of transcription 5A; STAT5; MGF |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | STAT5A |
| 全名 | signal transducer and activator of transcription 5A |
| 背景介绍 | Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription. |
| 生物功能 | Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30214 Phospho STAT5A Antibody Duo (Total, pY694) Related products: STAT5A antibodies; STAT5A Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| 研究领域 | Gene Regulation antibody; Signaling Transduction antibody |
| 预测分子量 | 91 kDa |
| 翻译后修饰 | Tyrosine phosphorylated in response to KITLG/SCF, IL2, IL3, IL7, IL15, CSF2/GMCSF, GH1, PRL, EPO and THPO. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity (By similarity). Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2 at Tyr-694. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates prolactin signaling pathway. ISGylated. |
检测图片 (2)
ARG51552 anti-STAT5A phospho (Tyr694) antibody WB image
Western blot: Extracts from K562 cells untreated or treated with G-CSF stained with ARG51552 anti-STAT5A phospho (Tyr694) antibody.
ARG51552 anti-STAT5A phospho (Tyr694) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51552 anti-STAT5A phospho (Tyr694) antibody (left) or the same antibody preincubated with blocking peptide (right).
参考文献
Effects of di (2-ethylhexyl) phthalate and high-fat diet on lipid metabolism in rats by JAK2/STAT5.
WB / Rat
Mono-2-ethylhexyl phthalate (MEHP) promoted lipid accumulation via JAK2/STAT5 and aggravated oxidative stress in BRL-3A cells.
WB / Rat
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