anti-TBP / TATA Binding Protein antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
-
Brand:
CAT.NO. : ARG41600
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Product Details
概述
| 产品描述 | Goat Polyclonal antibody recognizes TBP / TATA Binding Protein |
|---|---|
| 反应物种 | Hu, Ms, Rat, Pig |
| 预测物种 | Cow, Dog |
| 应用 | WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | TBP / TATA Binding Protein |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the internal region of Human TBP / TATA Binding Protein. (NP_003185.1, NP_001165556.1. C-TPQPIQNTNSLS) |
| 偶联标记 | Un-conjugated |
| 別名 | TATA-binding factor; SCA17; TATA sequence-binding protein; Transcription initiation factor TFIID TBP subunit; GTF2D1; TATA-box factor; TFIID; GTF2D; HDL4; TATA-box-binding protein |
应用说明
| 应用建议 |
| ||||
|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 实际分子量 | ~ 38 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TBP |
| 全名 | TATA box binding protein |
| 背景介绍 | Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 32-39, and expansion of the number of repeats increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2010] |
| 生物功能 | General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 38 kDa |
检测图片 (3)
ARG41600 anti-TBP / TATA Binding Protein antibody WB image
Western blot: 35 µg of HeLa (nuclear) and HeLa (cytosol) lysates (in RIPA buffer) stained with ARG41600 anti-TBP / TATA Binding Protein antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
ARG41600 anti-TBP / TATA Binding Protein antibody WB image
Western blot: 35 µg of NIH/3T3, Mouse testis and Rat testis lysates (in RIPA buffer) stained with ARG41600 anti-TBP / TATA Binding Protein antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
ARG41600 anti-TBP / TATA Binding Protein antibody WB image
Western blot: 35 µg of Pig testis lysate (in RIPA buffer) stained with ARG41600 anti-TBP / TATA Binding Protein antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
参考文献
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WB / Human
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