anti-Thymidine Kinase antibody [3B3.E11]
![anti-Thymidine Kinase antibody [3B3.E11]](/upload/image/20241105/8415bc38bc270b898ee2a90369a6c7fb.jpg)
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CAT.NO. : ARG54581
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [3B3.E11] recognizes Thymidine Kinase |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, WB |
| 特异性 | This antibody specifically recognizes human thymidine kinase. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 3B3.E11 |
| 同位型 | IgG1 |
| 靶点名称 | Thymidine Kinase |
| 抗原物种 | Human |
| 抗原 | 5’-His-tagged recombinanthuman thymidine kinase |
| 偶联标记 | Un-conjugated |
| 別名 | Thymidine kinase, cytosolic; TK2; EC 2.7.1.21 |
应用说明
| 应用说明 | Western blot: antibody at 10 ug/ml will recognize TK in 5, 10, or 20 ug lysates of cells that express TK. ELISA: use antibody at 2 - 5 ug/ml (optimized for TK on solid phase at 10 ug/ml). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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属性
| 形式 | Liquid |
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| 纯化 | Protein G-purified |
| 缓冲液 | PBS (pH 7.4) |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TK1 |
| 全名 | thymidine kinase 1, soluble |
| 背景介绍 | Thymidine kinase (TK) belongs to agroup of enzymes such as dihydrofolatereductase, thymidylate synthase, andDNA polymerase that are involved inDNA synthesis and precursorproduction. High levels of theseenzymes are present in proliferatingcells, and low levels are found in restingcells, due to multiple regulatorymechanisms that ensure exclusiveexpression of these enzymes inreplicating cells. TK is responsible forcatalyzing the phosphorylation ofthymidine, which functions as a part ofthe pyrimidine salvage pathway involvedin DNA synthesis. The activities ofenzymes such as TK are essential forthe activation of severalchemotherapeutically importantnucleoside analogues that areadministered as prodrugs. |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 25 kDa |
| 翻译后修饰 | Phosphorylated on Ser-13 in mitosis. |
参考文献
克隆号文献
Mechanism of action of the Aurora kinase inhibitor CCT129202 and in vivo quantification of biological activity.
Fluorodeoxyuridine modulates cellular expression of the DNA base excision repair enzyme uracil-DNA glycosylase.
WB / Human
IKK alpha regulates estrogen-induced cell cycle progression by modulating E2F1 expression.
Isolation and characterization of Dictyostelium thymidine kinase 1 as a calmodulin-binding protein.
WB / Dictyostelium discoideum
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