anti-TPH1 phospho (Ser260) antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG52459
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes TPH1 phospho (Ser260) |
|---|---|
| 反应物种 | Hu, Rat |
| 预测物种 | Ms, Bov, Chk, Dog, Zfsh |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | TPH1 |
| 抗原物种 | Rat |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser260 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | Tryptophan 5-hydroxylase 1; TRPH; EC 1.14.16.4; Tryptophan 5-monooxygenase 1; TPRH |
应用说明
| 应用建议 |
| ||||
|---|---|---|---|---|---|
| 应用说明 | Specific for the ~55k tryptophan hydroxylase protein phosphorylated at Ser260. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TPH1 |
| 全名 | tryptophan hydroxylase 1 |
| 背景介绍 | Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH (Jiang et al., 2000). |
| 研究领域 | Cancer antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 51 kDa |
检测图片 (1)
ARG52459 anti-TPH1 phospho (Ser260) antibody WB image
Western blot: Rat brainstem lysate showing specific immunolabeling of the ~55k TPH protein phosphorylated at Ser260 stained with ARG52459 anti-TPH1 phospho (Ser260) antibody. The labeling is specifically blocked by the phosphopeptide (Phos-pep) used as antigen. The corresponding non-phosphopeptide (Nonphos-pep) did not block the immunolabeling.
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