anti-UBE2L6 antibody [k1H3]
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Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
Product Details
| 产品描述 | Mouse Monoclonal antibody [k1H3] recognizes UBE2L6 |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | k1H3 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | UBE2L6 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-152 of Human UBE2L6. |
| 偶联标记 | Un-conjugated |
| 別名 | UbcH8; EC 6.3.2.19; Ubiquitin-protein ligase L6; UBCH8; Ubiquitin/ISG15-conjugating enzyme E2 L6; RIG-B; Ubiquitin carrier protein L6; Retinoic acid-induced gene B protein |
| 应用建议 |
| ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 0.1M Sodium citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | Swiss-port # O14933 Human Ubiquitin/ISG15-conjugating enzyme E2 L6 |
|---|---|
| 基因名称 | UBE2L6 |
| 全名 | ubiquitin-conjugating enzyme E2L 6 |
| 背景介绍 | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is highly similar in primary structure to the enzyme encoded by the UBE2L3 gene. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2011] |
| 生物功能 | Catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Promotes ubiquitination and subsequent proteasomal degradation of FLT3. [UniProt] |
| 预测分子量 | 18 kDa |
| 翻译后修饰 | ISGylated. |
ARG56963 anti-UBE2L6 antibody [k1H3] ICC/IF image
Immunoflorescense: HeLa cell line stained with ARG56963 anti-UBE2L6 antibody [k1H3] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
ARG56963 anti-UBE2L6 antibody [k1H3] IHC-P image
Immunohistochemistry: Paraffin embedded sections of Human breast lobule tissue stained with ARG56963 anti-UBE2L6 antibody [k1H3] at 1:50 for 2 hours at RT. Antigen Retrieval: Boil tissue section in 0.1M Sodium citrate buffer (pH 6.0) for 20 min.
ARG56963 anti-UBE2L6 antibody [k1H3] WB image
Western blot: 20 µg of MCF7 cell lysate stained with ARG56963 anti-UBE2L6 antibody [k1H3] at 1:2000.
ARG56963 anti-UBE2L6 antibody [k1H3] WB image
Western blot: 5 µg of 1) 293T cell lysate, 2) UBE2L6 Transfected 293T cell lysate stained with ARG56963 anti-UBE2L6 antibody [k1H3] at 1:2000.
ARG56963 anti-UBE2L6 antibody [k1H3] WB image
Western blot: 20 µg of Jurkat cell lysate stained with ARG56963 anti-UBE2L6 antibody [k1H3] at 1:2000.
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