anti-Ubiquitin antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG54630
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Ubiquitin |
|---|---|
| 反应物种 | Hu, Ms, Rat, Bov, Chk, Dm, Dog, E. coli, Fsh, Gpig, Hm, Mk, Pig, Rb, S. cerevisiae, Sheep, Xenopus laevis, Yeast |
| 应用 | ICC/IF, IE, IHC-Fr, IHC-P, IP, WB |
| 特异性 | This antibody will identify free ubiquitin aswell as ubiquinated proteins on western blot.It recognizes human, monkey, mouse, rat,hamster, rabbit guinea pig, bovine, porcine,canine, sheep, chicken, Xenopus, yeast,Drosophila, and fish ubiquitin. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Ubiquitin |
| 抗原物种 | Bovine |
| 抗原 | Native bovine ubiquitin conjugated to KLH. |
| 偶联标记 | Un-conjugated |
| 別名 | Polyubiquitin-B |
应用说明
| 应用建议 |
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| 应用说明 | IHC-Fr: For PFA perfusion fixed frozen section. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||||
| 阳性对照 | Heat-shocked HeLa and Mouse brain |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein A-purified antibody |
| 缓冲液 | PBS (pH 7.2), 0.09% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | UBB |
| 全名 | ubiquitin B |
| 背景介绍 | Ubiquitin is a highly-conserved, 76-aminoacid polypeptide expressed in all eukaryoticcells. Ubiquitination of proteins facilitatestargeting of cytosolic and nuclear proteinsfor degradation by proteasomes. Thisessential cellular process involves a multienzymecascade of ubiquitin-activatingenzymes (E1s), ubiquitin-conjugatingenzymes (E2s or Ubcs), and ubiquitinproteinligases (E3s). Ubiquitination alsoparticipates in internalization anddegradation of plasma membrane proteinssuch as T cell receptor subunits while stillassociated with membranes of theendoplasmic reticulum. |
| 生物功能 | Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. [UniProt] |
| 产品亮点 | Related products: Ubiquitin antibodies; Ubiquitin ELISA Kits; Ubiquitin Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| 研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody; Neuroscience antibody |
| 预测分子量 | 26 kDa |
| 翻译后修饰 | Ubiquitin: Phosphorylated at Ser-65 by PINK1 during mitophagy. Phosphorylated ubiquitin specifically binds and activates parkin (PRKN), triggering mitophagy (PubMed:24660806, PubMed:24751536, PubMed:24784582, PubMed:25527291). Phosphorylation does not affect E1-mediated E2 charging of ubiquitin but affects discharging of E2 enzymes to form polyubiquitin chains. It also affects deubiquitination by deubiquitinase enzymes such as USP30 (PubMed:25527291). |
检测图片 (1)
ARG54630 anti-Ubiquitin antibody WB image
Western Blot: Heat-shocked HeLa cell lysate stained with ARG54630 anti-Ubiquitin antibody.
参考文献
Targeting oncogenic MAGEA6 sensitizes triple negative breast cancer to doxorubicin through its autophagy and ferroptosis by stabling AMPKα1
WB / Human
Pro-prion, as a membrane adaptor protein for E3 ligase c-Cbl, facilitates the ubiquitination of IGF-1R, promoting melanoma metastasis
WB / Mouse
CD2-associated Protein Contributes to Hepatitis C Virus Propagation and Steatosis by Disrupting Insulin Signaling.
WB / Human
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