anti-Urokinase / uPA antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG63849
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Product Details
概述
| 产品描述 | Goat Polyclonal antibody recognizes Urokinase / uPA |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 特异性 | This antibody is expected to recognize both reported isoforms. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Urokinase / uPA |
| 抗原物种 | Human |
| 抗原 | C-HFLPWIRSHTKEEN |
| 偶联标记 | Un-conjugated |
| 別名 | ATF; uPA; U-plasminogen activator; BDPLT5; EC 3.4.21.73; QPD; URK; Urokinase-type plasminogen activator; u-PA; UPA |
应用说明
| 应用建议 |
| ||||
|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. Approx 48 kDa band observed in lysates of human kidney fibroblast cell line HEK293 (calculated MW of 48.5 kDa according to NP_002649.1). A minor band was also consistently observed at appox 35 kDa. This band was successfully blocked by incubation with the immunising peptide, and is consistent with the B chain of Cathepsin B-mediated cleavage product of PLAU (Kobayashi et al, J Biol Chem. 1991 Mar 15;266(8):5147-52; PMID: 1900515). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P00749 Human Urokinase-type plasminogen activator |
|---|---|
| 基因名称 | PLAU |
| 全名 | plasminogen activator, urokinase |
| 背景介绍 | This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer's disease and also with decreased affinity for fibrin-binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. Plasmin in turn cleaves this protein at a Lys-Ile bond to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009] |
| 生物功能 | Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody |
| 预测分子量 | 49 kDa |
| 翻译后修饰 | Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. |
检测图片 (1)
ARG63849 anti-Urokinase / uPA antibody WB image
Western blot: 35 µg of 293 cell lysate (in RIPA buffer) stained with ARG63849 anti-Urokinase / uPA antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
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