anti-VPS35 / MEM3 antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
CAT.NO. : ARG40916
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes VPS35 / MEM3 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | VPS35 / MEM3 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide derived from Human VPS35. |
| 偶联标记 | Un-conjugated |
| 別名 | Maternal-embryonic 3; Vacuolar protein sorting-associated protein 35; Vesicle protein sorting 35; PARK17; MEM3; hVPS35 |
应用说明
| 应用建议 |
| ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | HeLa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # Q96QK1 Human Vacuolar protein sorting-associated protein 35 Swiss-port # Q9EQH3 Mouse Vacuolar protein sorting-associated protein 35 |
|---|---|
| 基因名称 | VPS35 |
| 全名 | VPS35 retromer complex component |
| 背景介绍 | This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq, Jul 2008] |
| 生物功能 | Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The CSC seems to associate with the cytoplasmic domain of cargo proteins predominantly via VPS35; however, these interactions seem to be of low affinity and retromer SNX proteins may also contribute to cargo selectivity thus questioning the classical function of the CSC. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5 (Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R and SLC11A2. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Required for endosomal localization of FAM21C. Mediates the association of the CSC with the WASH complex via FAM21. Required for the endosomal localization of TBC1D5. [UniProt] |
| 细胞定位 | Cytoplasm. Membrane; Peripheral membrane protein. Endosome. Early endosome. Late endosome. Note=Localizes to tubular profiles adjacent to endosomes. [UniProt] |
| 预测分子量 | 92 kDa |
检测图片 (1)
ARG40916 anti-VPS35 / MEM3 antibody WB image
Western blot: HeLa cell lysate stained with ARG40916 anti-VPS35 / MEM3 antibody.
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