Biotin Anti-Mouse IgG1 Rabbit Monoclonal Antibody [Clone RM106], 31-1002-02
![Biotin Anti-Mouse IgG1 Rabbit Monoclonal Antibody [Clone RM106], 31-1002-02](/upload/image/products/RM106_WB-e1625768533710.png)
![Biotin Anti-Mouse IgG1 Rabbit Monoclonal Antibody [Clone RM106], 31-1002-02](/upload/image/products/Anri-mouse-IgG1-2.jpg)
![Biotin Anti-Mouse IgG1 Rabbit Monoclonal Antibody [Clone RM106], 31-1002-02](/upload/image/products/Anri-mouse-IgG1-3.jpg)
Key features and details
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Product Details
| Product Name | Biotin Anti-Mouse IgG1 Rabbit Monoclonal Antibody, Clone RM106 |
| Product Description | Biotinylated Rabbit monoclonal to Mouse IgG1 |
| Catalog No. | 31-1002-02 |
| Clone Name | RM106 |
| Product Data Sheet | Biotin Anti-Mouse IgG1 Rabbit Monoclonal Antibody Data Sheet RM106 |
| Specificity | RM106 reacts to the Fc region of mouse IgG1. No cross reactivity with mouse IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The Fc region of RM106 has been engineered to eliminate Fc receptor binding. |
| Immunogen | Mouse IgG |
| Application | ELISA Western Blot (WB) |
| Species Reactivity | Mouse |
| Conjugate | Biotin |
| Intended Use | Research Use Only |
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| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 50 μg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | ELISA: 0.005 μg/mL - 0.2 μg/mL; Western Blot (WB):0.1 μg/mL - 0.5 μg/mL. |
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| References | Liu F et al T cell-derived soluble glycoprotein GPIbα mediates PGE 2 production in human monocytes activated with the vaccine adjuvant MDP. Sci Signal. (2019), 10.1126/scisignal.aat6023. WB; Human. Read more (PubMed: 31594856) |
 | Western blot of nonreduced (-) and reduced (+) mouse IgG1 (20 ng/lane), using 0.2 μg/mL of RevMAb Clone RM106. This antibody reacts to nonreduced IgG1 (~150 kDa) stronger than the reduced γ1 form (~50 kDa). |
 | ELISA of mouse immunoglobulins shows RM106 reacts to the Fc region of mouse IgG1; no cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM106 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
 | A titer ELISA of mouse IgG1. The plate was coated with different amount of mouse IgG1. A serial dilution of RM106 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
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