cAMP-HRP [RM-PA466], 71-1358-00

Key features and details

Uniprot: N/A
Gene ID: N/A
Host Species: N/A
Species Reactivity: N/A
Concentration: N/A

Brand:

CAT.NO. : RM-PA466

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Size:

100µL

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Product Details


Product Name	cAMP-HRP (Peroxidase-Conjugated Cyclic Adenosine Monophosphate) [RM-PA466]
Catalog No.	RM-PA466 (71-1358-00)
Product Description	RevMAb cAMP-peroxidase conjugate is 8-[HRP]-AET-cAMP. It is suitable for RevMAb anti-cAMP rabbit monoclonal antibody (Clone RM466) in immunoassay development for cAMP detection.
Product Data Sheet	cAMP-HRP Datasheet
Application	ELISA
Intended Use	Research Use Only
Related Products	cAMP-HRP is antigen for Competitive Assay with Anti-cAMP Rabbit Monoclonal Antibody [RM466]
.

Properties

Form	Liquid
Volume/Item Size	100 µL
Storage Buffer	50% Glycerol/PBS with 1% BSA
Stability	Stable for 1 Year at -20.0°C from date of receipt
Usage	ELISA: 1:2,000 – 1:10,000 (The appropriate working concentrations may be affected by antibody affinity, the method of detection, temperature, the reaction time, and other factors.)
.

Product Images

Competitive ELISA of cAMP using anti-cAMP (RM466). The 96-well plate was coated
with 1 µg/mL of Goat anti-rabbit IgG (50 µL/well). 0.05 µg/mL of anti-cAMP (RM466) (50 µL/well)
was added and incubated. After wash and block, different concentrations of cAMP (25 µL/well)
were added along with 25 µL/well of 1/5,000 diluted HRP conjugated cAMP, 71-1358-00
TMB was used to develop the color after incubation and wash.

Competitive ELISA showing the specificity of anti-cAMP (RM466).
The 96-well plate was coated with 1 µg/mL of Goat anti-rabbit IgG (50 µL/well).
0.05 µg/mL of anti-cAMP (RM466) (50 µL/well) was added and incubated.
After wash and block, cAMP and other cyclic nucleotides or nucleoside
phosphates (25 µL/well) were added along with 25 µL/well of 1/5,000
diluted HRP conjugated cAMP, 71-1358-00. TMB was used to develop the
colorafter incubation and wash.

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