Glycolate Oxidase Assay Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记说明:
- 灵敏度:
- 检测范围:
- 样品类型:
- 样本量:
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Brand:
CAT.NO. : ARG82029
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Product Details
概述
| 产品描述 | ARG82029 Glycolate Oxidase Assay Kit is a detection kit for the quantification of Glycolate Oxidase Activity in tissue extracts, cell lysate and cell culture supernatants. |
|---|---|
| 反应物种 | All |
| 应用 | FuncSt |
| 靶点名称 | Glycolate Oxidase |
| 偶联标记说明 | Read at 500 nm. |
| 灵敏度 | 0.05 mmol/L |
| 检测范围 | 0.05 - 5 mmol/L |
| 样品类型 | Tissue extracts, cell lysate and cell culture supernatants. |
| 样本量 | 20 µl |
| 別名 | EC 1.1.3.15; Glycolate oxidase; HAOX1; GOX; GOX1; Hydroxyacid oxidase 1 |
应用说明
| 检测时间 | 15 min |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | HAO1 |
|---|---|
| 全名 | hydroxyacid oxidase (glycolate oxidase) 1 |
| 背景介绍 | This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq, Jul 2008] |
| 生物功能 | Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate. [UniProt] |
| 细胞定位 | Peroxisome. [UniProt] |
检测图片 (1)
ARG82029 Glycolate Oxidase Assay Kit typical data demonstration image
ARG82029 Glycolate Oxidase Assay Kit results of a typical data with optical density reading at 500 nm.
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