Goat anti-Mouse IgG antibody (Biotin), pre-adsorbed

Key features and details

  • 产品描述: Biotin-conjugated Goat Polyclonal antibody recognizes Mouse IgG
  • 反应物种: Ms
  • 应用: ELISA, ELISPOT, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, WB
  • 特异性: Reacts with the heavy chains of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3.
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: IgG
  • 抗原物种: Mouse
  • Brand:
CAT.NO. : ARG23487
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Product Details
概述
产品描述Biotin-conjugated Goat Polyclonal antibody recognizes Mouse IgG
反应物种Ms
应用ELISA, ELISPOT, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, WB
特异性Reacts with the heavy chains of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3.
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称IgG
抗原物种Mouse
抗原Mouse IgG
目标IgIgG
偶联标记Biotin
应用说明
预吸附Mouse IgM and IgA; Human immunoglobulins and pooled sera.
应用建议
应用推荐稀释比
ELISA1:5000 - 1:20000
ELISPOTAssay-dependent
FACS
FLISAAssay-dependent
ICC/IFAssay-dependent
IHC-Fr1:100 - 1:500
IHC-P1:250 - 1:1500
WBAssay-dependent
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液PBS and 0.1% Sodium azide.
抗菌剂0.1% Sodium azide
浓度0.5 mg/ml
存放说明Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
检测图片 (3)
  • ARG23487 Goat anti-Mouse IgG antibody (Biotin) (pre-adsorbed) ICC/IF image

    Immunofluorescence: Human epithelial carcinoma cell line HEp-2 stained with anti-Human CD44 antibody (right), followed by ARG23487 Goat anti-Mouse IgG antibody (Biotin) (pre-adsorbed), Streptavidin (FITC) and DAPI.

  • ARG23487 Goat anti-Mouse IgG antibody (Biotin) (pre-adsorbed) ELISA image

    ELISA: The plate was coated with purified Mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted ARG23487 Goat anti-Mouse IgG antibody (Biotin) (pre-adsorbed) followed by ARG23912 Streptavidin (HRP).

  • ARG23487 Goat anti-Mouse IgG antibody (Biotin) (pre-adsorbed) FACS image

    Flow Cytometry: Human peripheral blood lymphocytes stained with Mouse anti-Human CD3 antibody followed by ARG23487 Goat anti-Mouse IgG antibody (Biotin) (pre-adsorbed) followed by Streptavidin (FITC).

参考文献

Assembly of Interfacial Polyelectrolyte Complexation Fiber Bundles with Mineralization Gradient for Physiologically-inspired Ligament Regeneration

IHC-P / 

Tzu-Wei Wang et al.
,  (2023)

publication_link

 

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