Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed

Key features and details

  • 产品描述: Biotin-conjugated Goat Polyclonal antibody recognizes Mouse IgG2a
  • 反应物种: Ms
  • 应用: ELISA, ELISPOT, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, Puri, WB
  • 特异性: Reacts with the heavy chain of mouse IgG2a as demonstrated by ELISA, FLISA, and/or flow cytometry. Minimal cross reactivity with human immunoglobulins. May react with immunoglobulins from other species.
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: IgG2a
  • 抗原物种: Mouse
  • Brand:
CAT.NO. : ARG23806
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Product Details
概述
产品描述Biotin-conjugated Goat Polyclonal antibody recognizes Mouse IgG2a
反应物种Ms
应用ELISA, ELISPOT, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, Puri, WB
特异性Reacts with the heavy chain of mouse IgG2a as demonstrated by ELISA, FLISA, and/or flow cytometry. Minimal cross reactivity with human immunoglobulins. May react with immunoglobulins from other species.
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称IgG2a
抗原物种Mouse
偶联标记Biotin
应用说明
预吸附Mouse IgG1, IgG2b, IgG3, IgM, and IgA; Human immunoglobulins and pooled sera.
应用建议
应用推荐稀释比
ELISA1:5000 - 1:20000
ELISPOTAssay-dependent
FACS
FLISAAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
PuriAssay-dependent
WBAssay-dependent
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液PBS and 0.1% Sodium azide.
抗菌剂0.1% Sodium azide
浓度0.5 mg/ml
存放说明Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
检测图片 (4)
  • ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed ICC/IF image

    Immunofluorescence: TGFB1 stimulated Human dermal fibroblasts were stained with anti-ACTA2 followed by ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed, Cy3 conjugated Streptavidin, and DAPI.

  • ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed FACS image

    Flow Cytometry: Human blood peripheral lymphocytes were stained with Mouse anti-Human CD5 followed by ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed followed by Streptavidin-FITC.

  • ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed IHC image

    Immunohistochemistry: Paraffin-embedded Human colon cancer tissue was stained with ARG22191 anti-MMP9 antibody [SB15c] followed by ARG23806 Goat anti-Mouse IgG2a antibody (Biotin) (pre-adsorbed), ARG23912 Streptavidin (HRP), DAB, and hematoxylin.

  • ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed ELISA image

    ELISA: ELISA plate was coated with purified Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed followed by Streptavidin-HRP.

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