Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed
产品描述 | Biotin-conjugated Goat Polyclonal antibody recognizes Mouse IgG2a |
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反应物种 | Ms |
应用 | ELISA, ELISPOT, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, Puri, WB |
特异性 | Reacts with the heavy chain of mouse IgG2a as demonstrated by ELISA, FLISA, and/or flow cytometry. Minimal cross reactivity with human immunoglobulins. May react with immunoglobulins from other species. |
宿主 | Goat |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | IgG2a |
抗原物种 | Mouse |
偶联标记 | Biotin |
预吸附 | Mouse IgG1, IgG2b, IgG3, IgM, and IgA; Human immunoglobulins and pooled sera. | ||||||||||||||||||||
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应用建议 |
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应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
形式 | Liquid |
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纯化 | Affinity purification with immunogen. |
缓冲液 | PBS and 0.1% Sodium azide. |
抗菌剂 | 0.1% Sodium azide |
浓度 | 0.5 mg/ml |
存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed ICC/IF image
Immunofluorescence: TGFB1 stimulated Human dermal fibroblasts were stained with anti-ACTA2 followed by ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed, Cy3 conjugated Streptavidin, and DAPI.
ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed FACS image
Flow Cytometry: Human blood peripheral lymphocytes were stained with Mouse anti-Human CD5 followed by ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed followed by Streptavidin-FITC.
ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed IHC image
Immunohistochemistry: Paraffin-embedded Human colon cancer tissue was stained with ARG22191 anti-MMP9 antibody [SB15c] followed by ARG23806 Goat anti-Mouse IgG2a antibody (Biotin) (pre-adsorbed), ARG23912 Streptavidin (HRP), DAB, and hematoxylin.
ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed ELISA image
ELISA: ELISA plate was coated with purified Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed followed by Streptavidin-HRP.