HBV Core antigen / HBcAg ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG82006
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Size:
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Product Details
概述
| 产品描述 | ARG82006 HBV Core Antigen ELISA Kit is an Enzyme Immunoassay kit for the quantification of HBV Core Antigen in purified virus or unpurified viral supernatant. |
|---|---|
| 反应物种 | HBV |
| 应用 | ELISA |
| 特异性 | ARG82006 HBV Core Antigen ELISA Kit will recognize HBV core antigen from serotypes ADW, ADR, AYW and AYR. |
| 靶点名称 | HBV Core antigen / HBcAg |
| 偶联标记 | HRP |
| 偶联标记说明 | Read at 450 nm. |
| 灵敏度 | 1 ng/ml |
| 样品类型 | Purified virus or unpurified viral supernatant |
| 标准范围 | 1.56 - 100 ng/ml |
| 样本量 | 100 µl |
| 精确 | The CV values of intra-assay precision was 4-5% and inter-assay precision was 8%. |
| 別名 | precore; HBe antigen; PreC; HBeAg; precore protein; external core antigen; p25 |
应用说明
| 检测时间 | 4.5 hours |
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属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store components at 4°C or -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HBcAg |
| 全名 | Hepatitis B Virus Core antigen |
| 生物功能 | Self assembles to form an icosahedral capsid. Most capsid appear to be large particles with a icosahedral symmetry of T=4 and consist of 240 copies of capsid protein, though a fraction forms smaller T=3 particles consisting of 180 capsid proteins. Entering capsid are transported along microtubules to the nucleus. Phosphorylation of the capsid is thought to induce exposure of nuclear localization signal in the C-terminal portion of the capsid protein that allows binding to the nuclear pore complex via the importin (karyopherin-) alpha and beta. Capsids are imported in intact form through the nuclear pore into the nuclear basket, where it probably binds NUP153. Only capsids that contain the mature viral genome can release the viral DNA and capsid protein into the nucleoplasm. Immature capsids get stucked in the basket. Capsids encapsulate the pre-genomic RNA and the P protein. Pre-genomic RNA is reverse transcribed into DNA while the capsid is still in the cytoplasm. The capsid can then either be directed to the nucleus, providing more genome for transcription, or bud through the endoplasmic reticulum to provide new virions (By similarity). [UniProt] |
| 产品亮点 | Related products: HBV antibodies; HBV ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG82006 HBV Core antigen / HBcAg ELISA Kit standard curve image
ARG82006 HBV Core antigen / HBcAg ELISA Kit results of a typical standard run with optical density reading at 450 nm.
参考文献
Hepatitis B Virus Utilizes a Retrograde Trafficking Route via the Trans-Golgi Network to Avoid Lysosomal Degradation
ELISA / Virus / Cell culture supernatant
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