Human ARMET ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
- 标准范围:
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Brand:
CAT.NO. : ARG82404
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82404-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82404-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82404-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82404-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82404-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82404-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82404-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82404-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82404-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82404-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82404-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82404 Human ARMET ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ARMET in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | ARMET |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 46.8 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 93.7 - 6000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.8% Inter-Assay CV: 6.4% |
| 別名 | ARP; Arginine-rich protein; Protein ARMET; ARMET; Mesencephalic astrocyte-derived neurotrophic factor |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P55145 Human Mesencephalic astrocyte-derived neurotrophic factor |
|---|---|
| 基因名称 | MANF |
| 全名 | mesencephalic astrocyte-derived neurotrophic factor |
| 背景介绍 | The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related. [provided by RefSeq, Apr 2014] |
| 生物功能 | Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity). Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. [UniProt] |
| 细胞定位 | Secreted. Endoplasmic reticulum lumen. Sarcoplasmic reticulum lumen. Note=Retained in the endoplasmic reticulum (ER), and sarcoplasmic reticulum (SR) under normal conditions (PubMed:22637475). Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia (PubMed:22637475, PubMed:29497057). [UniProt] |
| 产品亮点 | Related products: ARMET antibodies; ARMET ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | May contain sialic acid residues. [UniProt] |
检测图片 (1)
ARG82404 Human ARMET ELISA Kit standard curve image
ARG82404 Human ARMET ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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