Human B4GALT1 ELISA Kit
CAT.NO. : ARG82407
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内含物
货号 | 内含物名称 | 包装 | 温度 |
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ARG82407-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
ARG82407-002 | Standard | 2 X 50 ng/vial | 4°C |
ARG82407-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
ARG82407-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
ARG82407-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
ARG82407-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
ARG82407-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
ARG82407-008 | 25X Wash buffer | 20 ml | 4°C |
ARG82407-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
ARG82407-010 | STOP solution | 10 ml (Ready to use) | 4°C |
ARG82407-011 | Plate sealer | 4 strips | Room temperature |
概述
产品描述 | ARG82407 Human B4GALT1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human B4GALT1 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
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反应物种 | Hu |
应用 | ELISA |
靶点名称 | B4GALT1 |
偶联标记 | HRP |
偶联标记说明 | Substrate: TMB and read at 450 nm. |
灵敏度 | 0.39 ng/ml |
样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
标准范围 | 0.78 - 50 ng/ml |
样本量 | 100 µl |
精确 | Intra-Assay CV: 5.5% Inter-Assay CV: 6.2% |
別名 | beta4Gal-T1; GTB; EC 2.4.1.38; Beta-1,4-GalTase 1; Beta4Gal-T1; Nal synthase; EC 2.4.1.22; Beta-1,4-galactosyltransferase 1; EC 2.4.1.-; B4GAL-T1; EC 2.4.1.90; GT1; b4Gal-T1; CDG2D; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1; GGTB2; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1 |
应用说明
检测时间 | ~ 5 hours |
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属性
形式 | 96 well |
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存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
数据库连接 | |
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基因名称 | B4GALT1 |
全名 | UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 |
背景介绍 | This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene is unique among the beta4GalT genes because it encodes an enzyme that participates both in glycoconjugate and lactose biosynthesis. For the first activity, the enzyme adds galactose to N-acetylglucosamine residues that are either monosaccharides or the nonreducing ends of glycoprotein carbohydrate chains. The second activity is restricted to lactating mammary tissues where the enzyme forms a heterodimer with alpha-lactalbumin to catalyze UDP-galactose + D-glucoseUDP + lactose. The two enzymatic forms result from alternate transcription initiation sites and post-translational processing. Two transcripts, which differ only at the 5' end, with approximate lengths of 4.1 kb and 3.9 kb encode the same protein. The longer transcript encodes the type II membrane-bound, trans-Golgi resident protein involved in glycoconjugate biosynthesis. The shorter transcript encodes a protein which is cleaved to form the soluble lactose synthase. [provided by RefSeq, Jul 2008] |
生物功能 | The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix. [UniProt] |
细胞定位 | Isoform Long: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. Cell surface. Cell projection, filopodium. Isoform Short: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. [UniProt] |
产品亮点 | Related products: B4GALT1 antibodies; B4GALT1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
翻译后修饰 | The soluble form derives from the membrane forms by proteolytic processing. [UniProt] |
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