Human B4GALT1 ELISA Kit

Key features and details

  • 产品描述: ARG82407 Human B4GALT1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human B4GALT1 in serum, plasma (EDTA, heparin) and cell culture supernatants.
  • 反应物种: Hu
  • 应用: ELISA
  • 靶点名称: B4GALT1
  • 偶联标记: HRP
  • 偶联标记说明: Substrate: TMB and read at 450 nm.
  • 灵敏度: 0.39 ng/ml
  • 样品类型: Serum, plasma (EDTA, heparin) and cell culture supernatants.
  • 标准范围: 0.78 - 50 ng/ml
  • Brand:
CAT.NO. : ARG82407
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Product Details
内含物
货号内含物名称包装温度
ARG82407-001Antibody-coated microplate8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82407-002Standard2 X 50 ng/vial 4°C
ARG82407-003Standard/Sample diluent30 ml (Ready to use) 4°C
ARG82407-004Antibody conjugate concentrate (100X)1 vial (100 µl) 4°C
ARG82407-005Antibody diluent buffer12 ml (Ready to use) 4°C
ARG82407-006HRP-Streptavidin concentrate (100X)1 vial (100 µl) 4°C
ARG82407-007HRP-Streptavidin diluent buffer12 ml (Ready to use) 4°C
ARG82407-00825X Wash buffer20 ml 4°C
ARG82407-009TMB substrate10 ml (Ready to use) 4°C (Protect from light)
ARG82407-010STOP solution10 ml (Ready to use) 4°C
ARG82407-011Plate sealer4 strips Room temperature
概述
产品描述ARG82407 Human B4GALT1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human B4GALT1 in serum, plasma (EDTA, heparin) and cell culture supernatants.
反应物种Hu
应用ELISA
靶点名称B4GALT1
偶联标记HRP
偶联标记说明Substrate: TMB and read at 450 nm.
灵敏度0.39 ng/ml
样品类型Serum, plasma (EDTA, heparin) and cell culture supernatants.
标准范围0.78 - 50 ng/ml
样本量100 µl
精确Intra-Assay CV: 5.5%
Inter-Assay CV: 6.2%
別名beta4Gal-T1; GTB; EC 2.4.1.38; Beta-1,4-GalTase 1; Beta4Gal-T1; Nal synthase; EC 2.4.1.22; Beta-1,4-galactosyltransferase 1; EC 2.4.1.-; B4GAL-T1; EC 2.4.1.90; GT1; b4Gal-T1; CDG2D; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1; GGTB2; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1
应用说明
检测时间~ 5 hours
属性
形式96 well
存放说明Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 2683 Human B4GALT1

Swiss-port # P15291 Human Beta-1,4-galactosyltransferase 1

基因名称B4GALT1
全名UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1
背景介绍This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene is unique among the beta4GalT genes because it encodes an enzyme that participates both in glycoconjugate and lactose biosynthesis. For the first activity, the enzyme adds galactose to N-acetylglucosamine residues that are either monosaccharides or the nonreducing ends of glycoprotein carbohydrate chains. The second activity is restricted to lactating mammary tissues where the enzyme forms a heterodimer with alpha-lactalbumin to catalyze UDP-galactose + D-glucoseUDP + lactose. The two enzymatic forms result from alternate transcription initiation sites and post-translational processing. Two transcripts, which differ only at the 5' end, with approximate lengths of 4.1 kb and 3.9 kb encode the same protein. The longer transcript encodes the type II membrane-bound, trans-Golgi resident protein involved in glycoconjugate biosynthesis. The shorter transcript encodes a protein which is cleaved to form the soluble lactose synthase. [provided by RefSeq, Jul 2008]
生物功能The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.

The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix. [UniProt]
细胞定位Isoform Long: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. Cell surface. Cell projection, filopodium. Isoform Short: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. [UniProt]
产品亮点Related products:
B4GALT1 antibodies; B4GALT1 ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
翻译后修饰The soluble form derives from the membrane forms by proteolytic processing. [UniProt]
检测图片 (1)
  • ARG82407 Human B4GALT1 ELISA Kit standard curve image

    ARG82407 Human B4GALT1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

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