Human Cathepsin D ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
-
Brand:
CAT.NO. : ARG81433
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Size:
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81433-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81433-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81433-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81433-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81433-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81433-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81433-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81433-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81433-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81433-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81433-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81433 Human Cathepsin D ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Cathepsin D in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | Cathepsin D |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.5% Inter-Assay CV: 7.5% |
| 別名 | CPSD; EC 3.4.23.5; HEL-S-130P; CLN10; Cathepsin D |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CTSD |
| 全名 | cathepsin D |
| 背景介绍 | This gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This proteinase, which is a member of the peptidase C1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites, including one which is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease. [provided by RefSeq, Jul 2008] |
| 生物功能 | Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. [UniProt] |
| 产品亮点 | Related products: Cathepsin antibodies; Cathepsin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | N- and O-glycosylated. Undergoes proteolytic cleavage and activation by ADAM30. As well as the major heavy chain which starts at Leu-169, 2 minor forms starting at Gly-170 and Gly-171 have been identified (PubMed:1426530). An additional form starting at Ala-168 has also been identified (PubMed:27333034). [UniProt] |
检测图片 (1)
ARG81433 Human Cathepsin D ELISA Kit standard curve image
ARG81433 Human Cathepsin D ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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