Human CD169 / Siglec 1 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
-
Brand:
CAT.NO. : ARG81551
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81551-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81551-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81551-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81551-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81551-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81551-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81551-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81551-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81551-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81551-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81551-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81551 Human CD169 / Siglec 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD169 / Siglec 1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | CD169 / Siglec 1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.9% Inter-Assay CV: 7.9% |
| 別名 | CD169; Siglec-1; dJ1009E24.1; Sialic acid-binding Ig-like lectin 1; SIGLEC-1; CD antigen CD169; SN; Sialoadhesin |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SIGLEC1 |
| 全名 | sialic acid binding Ig-like lectin 1, sialoadhesin |
| 背景介绍 | This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008] |
| 生物功能 | Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis. [UniProt] |
| 产品亮点 | Related products: CD169 antibodies; CD169 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG81551 Human CD169 / Siglec 1 ELISA Kit standard curve image
ARG81551 Human CD169 / Siglec 1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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