Human CD170 / Siglec 5 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
-
Brand:
CAT.NO. : ARG81972
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Size:
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81972-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81972-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81972-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81972-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81972-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81972-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81972-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81972-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81972-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81972-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81972-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81972 Human CD170 / Siglec 5 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD170 / Siglec 5 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | CD170 / Siglec 5 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 23.45 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 46.9 - 3000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.6%; Inter-Assay CV: 6.4% |
| 別名 | OBBP2; OB-binding protein 2; CD170; Sialic acid-binding Ig-like lectin 5; SIGLEC-5; CD antigen CD170; CD33 antigen-like 2; OB-BP2; CD33L2; Siglec-5; Obesity-binding protein 2 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # O15389 Human Sialic acid-binding Ig-like lectin 5 |
|---|---|
| 基因名称 | SIGLEC5 |
| 全名 | sialic acid binding Ig-like lectin 5 |
| 背景介绍 | This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion. [provided by RefSeq, Feb 2012] |
| 生物功能 | Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. [UniProt] |
| 产品亮点 | Related products: CD170 antibodies; CD170 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG81972 Human CD170 / Siglec 5 ELISA Kit standard curve image
ARG81972 Human CD170 / Siglec 5 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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