Human IL-12B p40 ELISA Kit

Key features and details

Specification: 96 Test
Sensitivity: 0.29 pg/ml (50 μl)；1.84 pg/ml (10 μl)
Standard Curve Range: 1.37~1000 pg/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0098

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Size:

96T

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Product Details

Background

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

Tipical Data

pg/ml	O.D.		Average	Corrected
0.00	0.0228	0.0235	0.0232
1.37	0.0310	0.0304	0.0307	0.0076
4.12	0.0481	0.0481	0.0481	0.0250
12.35	0.0945	0.0931	0.0938	0.0707
37.04	0.2360	0.2307	0.2334	0.2102
111.11	0.6390	0.6322	0.6356	0.6125
333.33	1.7120	1.6570	1.6845	1.6614
1000.00	3.8910	3.8380	3.8645	3.8414

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average(pg/ml)	19.4	104.0	339.2	19.3	101.1	309.6
Standard Deviation	0.7	4.3	14.4	1.3	5.4	20.1
Coefficient of Variation(%)	3.4	4.1	4.2	6.6	5.4	6.5

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-12B p40 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 117% with an overall mean recovery of 102%.

Sample Values

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	25.15-193.73	100	80.27

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. Donor medical histories were not available.

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