Human CD47 recombinant protein (ECD) (Fc-His-tagged, C-ter)
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 靶点名称:
- 物种:
- A.A. 序列:
- 表达系统:
- 別名:
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Brand:
CAT.NO. : ARG70294
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Product Details
概述
| 产品描述 | HEK293 expressed, Fc-His-tagged (C-ter) Human CD47 recombinant protein (ECD). |
|---|---|
| 反应物种 | Hu |
| 应用 | Binding, SDS-PAGE |
| 靶点名称 | CD47 (ECD) |
| 物种 | Human |
| A.A. 序列 | Gln19 - Pro139 of Human CD47 (NP_001768.1) with an Fc-6X His tag at the C-terminus. |
| 表达系统 | HEK293 |
| 別名 | Leukocyte surface antigen CD47; CD antigen CD47; Antigenic surface determinant protein OA3; MER6; OA3; Protein MER6; IAP; Integrin-associated protein |
应用说明
| 应用说明 | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human CD47 at 1 µg/ml (100 µl/well) can bind Recombinant Human SIRP alpha with a linear range of 1-6 ng/ml. |
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属性
| 形式 | Powder |
|---|---|
| 纯化说明 | 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 97% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 复溶 | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD47 |
| 全名 | CD47 molecule |
| 背景介绍 | This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010] |
| 生物功能 | Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. [UniProt] |
| 细胞定位 | Cell membrane; Multi-pass membrane protein. [UniProt] |
| 预测分子量 | 35 kDa |
检测图片 (1)
ARG70294 Human CD47 recombinant protein (ECD) (Fc-His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70294 Human CD47 recombinant protein (ECD) (Fc-His-tagged, C-ter).
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