Human CNTN1 / Contactin 1 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
- 标准范围:
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Brand:
CAT.NO. : ARG82386
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82386-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82386-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82386-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82386-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82386-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82386-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82386-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82386-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82386-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82386-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82386-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82386 Human CNTN1 / Contactin 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CNTN1 / Contactin 1 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | CNTN1 / Contactin 1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.6% Inter-Assay CV: 7.2% |
| 別名 | Contactin-1; F3; Glycoprotein gp135; GP135; Neural cell surface protein F3; MYPCN |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CNTN1 |
| 全名 | contactin 1 |
| 背景介绍 | The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
| 生物功能 | Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth (By similarity). [UniProt] |
| 细胞定位 | Isoform 1: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. Isoform 2: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. [UniProt] |
| 产品亮点 | Related products: Contactin antibodies; Contactin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG82386 Human CNTN1 / Contactin 1 ELISA Kit standard curve image
ARG82386 Human CNTN1 / Contactin 1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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