Human Cystatin B / Stefin B ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
- 标准范围:
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Brand:
CAT.NO. : ARG82068
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82068-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82068-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82068-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82068-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82068-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82068-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82068-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82068-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82068-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82068-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82068-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | Human Cystatin B / Stefin B ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Cystatin B / Stefin B in serum, plasma (heparin, EDTA), saliva, urine, human milk and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | Cystatin B / Stefin B |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 31.25 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA), saliva, urine, human milk and cell culture supernatants. |
| 标准范围 | 62.5 - 4000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 7.4%; Inter-Assay CV: 8.2% |
| 別名 | Liver thiol proteinase inhibitor; EPM1; CPI-B; EPM1A; Cystatin-B; Stefin-B; PME; CST6; ULD; STFB |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CSTB |
| 全名 | cystatin B (stefin B) |
| 背景介绍 | The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). [provided by RefSeq, Jul 2008] |
| 生物功能 | This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. [UniProt] |
| 产品亮点 | Related products: Cystatin antibodies; Cystatin ELISA Kits; Cystatin Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG82068 Human Cystatin B / Stefin B ELISA Kit standard curve image
ARG82068 Human Cystatin B / Stefin B ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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