Human ERBB3 / HER3 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81570
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81570-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81570-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81570-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81570-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81570-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81570-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81570-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81570-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81570-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81570-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81570-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81570 Human ERBB3 / HER3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ERBB3 / HER3 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | ERBB3 / HER3 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.8% Inter-Assay CV: 6.8% |
| 別名 | MDA-BF-1; LCCS2; p180-ErbB3; Proto-oncogene-like protein c-ErbB-3; c-erbB3; p85-sErbB3; Tyrosine kinase-type cell surface receptor HER3; p45-sErbB3; erbB3-S; HER3; c-erbB-3; ErbB-3; EC 2.7.10.1; Receptor tyrosine-protein kinase erbB-3 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P21860 Human Receptor tyrosine-protein kinase erbB-3 |
|---|---|
| 基因名称 | ERBB3 |
| 全名 | erb-b2 receptor tyrosine kinase 3 |
| 背景介绍 | This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq, Jul 2008] |
| 生物功能 | Binds and is activated by neuregulins and NTAK. May also be activated by CSPG5. [UniProt] |
| 产品亮点 | Related products: ERBB3 antibodies; ERBB3 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Autophosphorylated (PubMed:20351256). Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (PubMed:20682778). [UniProt] |
检测图片 (1)
ARG81570 Human ERBB3 / HER3 ELISA Kit standard curve image
ARG81570 Human ERBB3 / HER3 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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