Human Growth hormone receptor ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81582
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81582-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81582-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81582-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81582-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81582-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81582-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81582-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81582-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81582-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81582-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81582-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81582 Human Growth hormone receptor ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Growth hormone receptor in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | Growth hormone Receptor |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.3% Inter-Assay CV: 5.9% |
| 別名 | GH-binding protein; Growth hormone receptor; GHBP; GHIP; Serum-binding protein; GH receptor; Somatotropin receptor |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | GHR |
| 全名 | growth hormone receptor |
| 背景介绍 | This gene encodes a member of the type I cytokine receptor family, which is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. In humans and rabbits, but not rodents, growth hormone binding protein (GHBP) is generated by proteolytic cleavage of the extracellular ligand-binding domain from the mature growth hormone receptor protein. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011] |
| 生物功能 | Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway (By similarity). The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling. Isoform 2 up-regulates the production of GHBP and acts as a negative inhibitor of GH signaling. [UniProt] |
| 产品亮点 | Related products: Growth hormone Receptor antibodies; Growth hormone Receptor ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. Shedding is inhibited by growth hormone (GH) binding to the receptor probably due to a conformational change in GHR rendering the receptor inaccessible to ADAM17 (By similarity). On GH binding, phosphorylated on tyrosine residues in the cytoplasmic domain by JAK2. On ligand binding, ubiquitinated on lysine residues in the cytoplasmic domain. This ubiquitination is not sufficient for GHR internalization (By similarity). [UniProt] |
检测图片 (1)
ARG81582 Human Growth hormone receptor ELISA Kit standard curve image
ARG81582 Human Growth hormone receptor ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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