Human IFN-α ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Gradient:
- Number of Incubations:
- Assay type:
-
Brand:
CAT.NO. : AEH0085
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Size:
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Product Details
Background
Interferon-alpha (IFN-α) is a protein produced by the immune system in response to viral infections. It's a type of cytokine that's part of the innate immune response and has many biological functions, including:
1.Antiviral effects: IFN-α inhibits the replication of viruses in infected cells ;
2.Immunomodulatory effects: IFN-α enhances the expression of major histocompatibility complex (MHC) antigens and regulates cell differentiation ;
3.Cancer treatment: IFN-α is used to treat a number of tumors and is used in combination with other antiviral drugs ;
IFN-α is produced by leukocytes and binds to the IFN-α receptor (IFNAR). It's used to treat viral infections like hepatitis B and C, and a number of tumors. IFN-α is also used as a pharmaceutical drug called Multiferon, which is made from the leukocyte fraction of human blood.
1.Antiviral effects: IFN-α inhibits the replication of viruses in infected cells ;
2.Immunomodulatory effects: IFN-α enhances the expression of major histocompatibility complex (MHC) antigens and regulates cell differentiation ;
3.Cancer treatment: IFN-α is used to treat a number of tumors and is used in combination with other antiviral drugs ;
IFN-α is produced by leukocytes and binds to the IFN-α receptor (IFNAR). It's used to treat viral infections like hepatitis B and C, and a number of tumors. IFN-α is also used as a pharmaceutical drug called Multiferon, which is made from the leukocyte fraction of human blood.
TYPICAL DATA
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0 |
0.0187 |
0.0181 |
0.0184 |
|
|
0.41 |
0.0407 |
0.0408 |
0.0408 |
0.0224 |
|
1.23 |
0.0767 |
0.0753 |
0.076 |
0.0576 |
|
3.7 |
0.1796 |
0.1758 |
0.1777 |
0.1593 |
|
11.11 |
0.4858 |
0.484 |
0.4849 |
0.4665 |
|
33.33 |
1.226 |
1.234 |
1.23 |
1.2116 |
|
100 |
2.561 |
2.595 |
2.578 |
2.5596 |
|
300 |
4.2605 |
4.2605 |
4.2605 |
4.2421 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average（ng/ml） |
11.4 |
51.2 |
155.6 |
12 |
57 |
175 |
|
Standard Deviation |
0.5 |
3.8 |
9.8 |
0.7 |
1.8 |
4.2 |
|
Coefficient Of Variation（%） |
4.4 |
7.3 |
6.3 |
5.3 |
3.1 |
2.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IFN-alpha into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 128% with an overall mean recovery of 98%.
The recovery ranged from 85% to 128% with an overall mean recovery of 98%.
Sample Values
|
Sample Matrix |
Sample Evaluated |
Range(ng/ml) |
Detectable% |
Mean of Detectable(ng/ml) |
|
Serum |
30 |
n.d.-0.9 |
31.3 |
0.21 |
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
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