Human IL4 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG83359
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG83359-01 | Antibody Coated Microplate | 8 X 12 strips | 4°C |
| ARG83359-02 | Standards Mixture | 3 vials | 4°C |
| ARG83359-03 | 100X Antibody Conjugate Mixture | 120 μl | ≤ -20°C |
| ARG83359-04 | 1000X HRP-Streptavidin solution | 15 μl | 4°C |
| ARG83359-05 | Standard/Sample Diluent Buffer | 20 ml | 4°C |
| ARG83359-06 | Antibody Diluent Buffer | 30 ml | 4°C |
| ARG83359-07 | 10X Wash Buffer | 50 ml | 4°C |
| ARG83359-08 | TMB substrate | 12 ml | 4°C (protect from light) |
| ARG83359-09 | STOP solution | 12 ml | 4°C |
| ARG83359-10 | Plate sealer | 4 adhesive strips | Room temperature |
概述
| 产品描述 | ARG83359 Human IL4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL4 in Serum, Plasma, Cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | No significant cross-reactivity or interference with IL1, IL2, IL3, IL5, IL6, IL8, IL10, IL12, IFN, TNF and sTNF-RII |
| 靶点名称 | IL4 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm |
| 灵敏度 | 3.9 pg/ml |
| 样品类型 | Serum, Plasma, Cell culture supernatants |
| 标准范围 | 7.8 - 500 pg/ml |
| 样本量 | 50 µl |
| 精确 | CV: less than10% |
| 別名 | BSF-1; IL-4; B-cell stimulatory factor 1; BCGF1; Lymphocyte stimulatory factor 1; BCGF-1; Interleukin-4; Pitrakinra; Binetrakin; BSF1 |
应用说明
| 检测时间 | 4 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | IL4 |
| 全名 | interleukin 4 |
| 背景介绍 | The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| 生物功能 | Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. [UniProt] |
| 产品亮点 | Related products: IL4 antibodies; IL4 ELISA Kits; IL4 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 研究领域 | Cancer kit; Immune System kit |
检测图片 (1)
ARG83359 Human IL4 ELISA Kit standard curve image
ARG83359 Human IL4 ELISA Kitresults of a typical standard run with optical density reading at 450 nm.
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