Human MMP7 (total) ELISA Kit
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Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
- 标准范围:
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Brand:
CAT.NO. : ARG82009
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82009-001 | Antibody-coated microplate | 12 X 8 strips | 4°C |
| ARG82009-002 | Standard (200 ng) | 1 vial | 4°C, store at -20°C after reconstitution |
| ARG82009-003 | Standard reconstitution buffer | 1.1 ml | 4°C |
| ARG82009-004 | HRP-antibody Conjugate (200X) | 65 µl | 4°C |
| ARG82009-005 | Sample diluent | 15 ml | 4°C |
| ARG82009-006 | Assay buffer | 12 ml | 4°C |
| ARG82009-007 | Antibody diluent | 12 ml | 4°C |
| ARG82009-008 | 10X Wash buffer | 30 ml | 4°C |
| ARG82009-009 | TMB substrate | 12 ml (ready-to-use) | 4°C (Protect from light) |
| ARG82009-010 | Stop solution | 6 ml | 4°C |
| ARG82009-011 | Plate sealer | 1 strip | Room temperature |
概述
| 产品描述 | ARG82009 Human MMP7 (total) ELISA Kit is an Enzyme Immunoassay kit for the quantification of human total MMP7 in serum, plasma, saliva, urine, cell lysate and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | MMP7 (total) |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 0.21 ng/ml |
| 样品类型 | Serum, plasma, saliva, urine, cell lysate and cell culture supernatants. |
| 标准范围 | 0.3125 - 20 ng/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.9%; Inter-Assay CV: 6.6% |
| 別名 | MMP-7; Uterine metalloproteinase; Matrix metalloproteinase-7; EC 3.4.24.23; Matrilysin; Matrin; Pump-1 protease; PUMP-1; MPSL1 |
应用说明
| 检测时间 | ~ 4 hours |
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属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MMP7 |
| 全名 | matrix metallopeptidase 7 |
| 背景介绍 | MMP7 is a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal hemopexin domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes on chromosome 11. This gene exhibits elevated expression levels in multiple human cancers. [provided by RefSeq, Jan 2016] |
| 生物功能 | MMP7 degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase. [UniProt] |
| 细胞定位 | Secreted, extracellular space, extracellular matrix. [UniProt] |
| 产品亮点 | Related products: MMP7 antibodies; MMP7 ELISA Kits; Related news: New MMP7 (total or active) ELISA kits are released New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (2)
ARG82009 Human MMP7 (total) ELISA Kit standard curve image
ARG82009 Human MMP7 (total) ELISA Kit results of a typical standard run with optical density reading at 450 nm.
ARG82009 Human MMP7 (total) ELISA Kit standard curve image
ARG82009 Human MMP7 (total) ELISA Kit results of a typical standard run with optical density reading at 450 nm. Red:Mature MMP7; Green: Pro-MMP7.
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