Human MUC16 / CA125 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
-
Brand:
CAT.NO. : ARG82620
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82620-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82620-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82620-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82620-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82620-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82620-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82620-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82620-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82620-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82620-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82620-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82620 Human MUC16 / CA125 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MUC16 / CA125 in Humanserum, plasma (EDTA, heparin), cell culture supernatants, saliva, urine and milk. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | MUC16 / CA125 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 10 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin), cell culture supernatants, saliva, urine and milk. |
| 标准范围 | 15.6 - 1000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.5% Inter-Assay CV: 6.2% |
| 別名 | Mucin-16; MUC-16; Ovarian carcinoma antigen CA125; CA125; Ovarian cancer-related tumor marker CA125; CA-125 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MUC16 |
| 全名 | mucin 16, cell surface associated |
| 生物功能 | Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. Secreted, extracellular space. Note=May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain. [UniProt] |
| 产品亮点 | Related products: MUC16 antibodies; MUC16 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Heavily O-glycosylated; expresses both type 1 and type 2 core glycans. Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans. May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space. May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts. [UniProt] |
检测图片 (1)
ARG82620 Human MUC16 / CA125 ELISA Kit standard curve image
ARG82620 Human MUC16 / CA125 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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