Human PCOLCE / PCPE1 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
- 标准范围:
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Brand:
CAT.NO. : ARG82749
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Size:
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82749-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82749-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82749-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82749-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82749-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82749-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82749-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82749-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82749-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82749-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82749-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82749 Human PCOLCE / PCPE1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human PCOLCE / PCPE1 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | PCOLCE / PCPE1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.3% Inter-Assay CV: 6.2% |
| 別名 | Type I procollagen COOH-terminal proteinase enhancer; Procollagen C-endopeptidase enhancer 1; Procollagen C-proteinase enhancer 1; PCPE-1; Type 1 procollagen C-proteinase enhancer protein; PCPE1; Procollagen COOH-terminal proteinase enhancer 1; PCPE |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # Q15113 Human Procollagen C-endopeptidase enhancer 1 |
|---|---|
| 基因名称 | PCOLCE |
| 全名 | procollagen C-endopeptidase enhancer |
| 背景介绍 | Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity. [provided by RefSeq, Jul 2008] |
| 生物功能 | Binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity. C-terminal processed part of PCPE (CT-PCPE) may have an metalloproteinase inhibitory activity. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 产品亮点 | Related products: PCOLCE ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | C-terminally processed at multiple positions. [UniProt] |
检测图片 (1)
ARG82749 Human PCOLCE / PCPE1 ELISA Kit standard curve image
ARG82749 Human PCOLCE / PCPE1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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