Human PON1 / paraoxonase 1 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81509
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81509-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81509-002 | Standard | 2 X 2 ng/vial | 4°C |
| ARG81509-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81509-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81509-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81509-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81509-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81509-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81509-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81509-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81509-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81509 Human PON1 / paraoxonase 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human PON1 / paraoxonase 1 in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | PON1 / paraoxonase 1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (heparin) and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.2% Inter-Assay CV: 5.8% |
| 別名 | K-45; Aromatic esterase 1; MVCD5; EC 3.1.1.81; A-esterase 1; PON; Serum paraoxonase/arylesterase 1; ESA; Serum aryldialkylphosphatase 1; EC 3.1.8.1; EC 3.1.1.2; PON 1 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PON1 |
| 全名 | paraoxonase 1 |
| 背景介绍 | The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3. [provided by RefSeq, Oct 2008] |
| 生物功能 | Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. [UniProt] |
| 产品亮点 | Related products: PON1 antibodies; PON1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Glycosylated. The signal sequence is not cleaved. Present in two forms, form B contains a disulfide bond, form A does not. [UniProt] |
检测图片 (1)
ARG81509 Human PON1 / paraoxonase 1 ELISA Kit standard curve image
ARG81509 Human PON1 / paraoxonase 1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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