Human SERPING1 / C1 Inhibitor ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
-
Brand:
CAT.NO. : ARG81626
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81626-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81626-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81626-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81626-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81626-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81626-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81626-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81626-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81626-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81626-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81626-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81626 Human SERPING1 / C1 Inhibitor ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human SERPING1 / C1 Inhibitor in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | SERPING1 / C1 Inhibitor |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.7% Inter-Assay CV: 6.8% |
| 別名 | C1IN; C1INH; C1-inhibiting factor; Serpin G1; C1 esterase inhibitor; Plasma protease C1 inhibitor; C1 Inh; HAE2; C1Inh; C1NH; HAE1 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SERPING1 |
| 全名 | serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 |
| 背景介绍 | This gene encodes a highly glycosylated plasma protein involved in the regulation of the complement cascade. Its protein inhibits activated C1r and C1s of the first complement component and thus regulates complement activation. Deficiency of this protein is associated with hereditary angioneurotic oedema (HANE). Alternative splicing results in multiple transcript variants encoding the same isoform. [provided by RefSeq, Jul 2008] |
| 生物功能 | Activation of the C1 complex is under control of the C1-inhibitor. It forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. Inhibits chymotrypsin and kallikrein. [UniProt] |
| 产品亮点 | Related products: Serpin antibodies; Serpin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Highly glycosylated (49%) with N- and O-glycosylation. O-glycosylated with core 1 or possibly core 8 glycans. N-glycan heterogeneity at Asn-25: Hex5HexNAc4 (minor), dHex1Hex5HexNAc4 (minor), Hex6HexNAc5 (major) and dHex1Hex6HexNAc5 (minor). Can be proteolytically cleaved by E.coli stcE. [UniProt] |
检测图片 (1)
ARG81626 Human SERPING1 / C1 Inhibitor ELISA Kit standard curve image
ARG81626 Human SERPING1 / C1 Inhibitor ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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