Human Smac / Diablo ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
- 标准范围:
-
Brand:
CAT.NO. : ARG82845
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82845-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82845-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82845-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82845-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82845-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82845-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82845-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82845-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82845-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82845-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82845-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82845 Human Smac / Diablo ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Smac / Diablo in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | Smac / Diablo |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.5% Inter-Assay CV: 7.6% |
| 別名 | Smac; Second mitochondria-derived activator of caspase; Diablo homolog, mitochondrial; SMAC; Direct IAP-binding protein with low pI; DFNA64 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | DIABLO |
| 全名 | diablo, IAP-binding mitochondrial protein |
| 背景介绍 | This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013] |
| 生物功能 | Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance. [UniProt] |
| 细胞定位 | Mitochondrion. Note=Released into the cytosol when cells undergo apoptosis. [UniProt] |
| 翻译后修饰 | Ubiquitinated by BIRC7/livin. [UniProt] |
检测图片 (1)
ARG82845 Human Smac / Diablo ELISA Kit standard curve image
ARG82845 Human Smac / Diablo ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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