Human Surfactant protein D ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81518
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81518-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81518-002 | Standard | 2 X 40 ng/vial | 4°C |
| ARG81518-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81518-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81518-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81518-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81518-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81518-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81518-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81518-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81518-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81518 Human Surfactant protein D ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Surfactant protein D in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | Surfactant protein D |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 312.5 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 625 - 40000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.3% Inter-Assay CV: 7.3% |
| 別名 | SP-D; COLEC7; Lung surfactant protein D; SFTP4; Pulmonary surfactant-associated protein D; PSP-D; Collectin-7 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P35247 Human Pulmonary surfactant-associated protein D |
|---|---|
| 基因名称 | SFTPD |
| 全名 | surfactant protein D |
| 背景介绍 | The protein encoded by this gene is part of the innate immune response, protecting the lungs against inhaled microorganisms and chemicals. The encoded protein may also be involved in surfactant metabolism. [provided by RefSeq, Jul 2015] |
| 生物功能 | Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. [UniProt] |
| 产品亮点 | Related products: Surfactant protein D ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | The N-terminus is blocked. Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates. S-nitrosylation at Cys-35 and Cys-40 alters the quaternary structure which results in a pro-inflammatory chemoattractive signaling activity with macrophages. [UniProt] |
检测图片 (1)
ARG81518 Human Surfactant protein D ELISA Kit standard curve image
ARG81518 Human Surfactant protein D ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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