Human WIF1 ELISA Kit

产品描述: ARG81598 Human WIF1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human WIF1 in serum, plasma (heparin, EDTA) and cell culture supernatants.
反应物种: Hu
应用: ELISA
特异性: There is no detectable cross-reactivity with other relevant proteins.
靶点名称: WIF1
偶联标记: HRP
偶联标记说明: Substrate: TMB and read at 450 nm.
灵敏度: 11.7 pg/ml
样品类型: Serum, plasma (heparin, EDTA) and cell culture supernatants.

CAT.NO. : ARG81598

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Size:

96 wells

Product Details

内含物

货号	内含物名称	包装	温度
ARG81598-001	Antibody-coated microplate	8 X 12 strips	4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81598-002	Standard	2 X 10 ng/vial	4°C
ARG81598-003	Standard/Sample diluent	30 ml (Ready to use)	4°C
ARG81598-004	Antibody conjugate concentrate (100X)	1 vial (130 µl)	4°C
ARG81598-005	Antibody diluent buffer	12 ml (Ready to use)	4°C
ARG81598-006	HRP-Streptavidin concentrate (100X)	1 vial (130 µl)	4°C
ARG81598-007	HRP-Streptavidin diluent buffer	12 ml (Ready to use)	4°C
ARG81598-008	25X Wash buffer	20 ml	4°C
ARG81598-009	TMB substrate	10 ml (Ready to use)	4°C (Protect from light)
ARG81598-010	STOP solution	10 ml (Ready to use)	4°C
ARG81598-011	Plate sealer	4 strips	Room temperature

概述

产品描述	ARG81598 Human WIF1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human WIF1 in serum, plasma (heparin, EDTA) and cell culture supernatants.
反应物种	Hu
应用	ELISA
特异性	There is no detectable cross-reactivity with other relevant proteins.
靶点名称	WIF1
偶联标记	HRP
偶联标记说明	Substrate: TMB and read at 450 nm.
灵敏度	11.7 pg/ml
样品类型	Serum, plasma (heparin, EDTA) and cell culture supernatants.
标准范围	23.4 - 1500 pg/ml
样本量	100 µl
精确	Intra-Assay CV: 5.1% Inter-Assay CV: 6.9%
別名	WIF-1; Wnt inhibitory factor 1

应用说明

检测时间	~ 5 hours

属性

形式	96 well
存放说明	Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 11197 Human WIF1 Swiss-port # Q9Y5W5 Human Wnt inhibitory factor 1
基因名称	WIF1
全名	WNT inhibitory factor 1
背景介绍	The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers. [provided by RefSeq, Jun 2010]
生物功能	Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation. [UniProt]
产品亮点	Related products: WIF1 antibodies; WIF1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData

检测图片 (1)

ARG81598 Human WIF1 ELISA Kit standard curve image
ARG81598 Human WIF1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

参考文献

Epigenetic regulation of human WIF1 and DNA methylation situation of WIF1 and GSTM5 in urothelial carcinoma

ELISA / Human/Cell culture supernatants

Cheng-Huang Shen et al.
Heliyon., (2023)