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Isotype Controls

An isotype control antibody, a primary antibody used as a negative control, helps determine the non - specific background staining level in an experiment.

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Function:

It differentiates between non - specific background and specific antibody signals. It indicates whether a sample has proper blocking and washing or needs further optimization.

Characteristics:

It has the same immunoglobulin isotype and subclass as the primary antibody but lacks target specificity. It's specific to a known irrelevant target like keyhole limpet hemocyanin (KLH).

Applications:

Mainly used in flow cytometry, it can also be a blocking or protein - coating agent in immunofluorescence, immunocytochemistry, Western blotting, and ELISA.

Selection:

When selecting an isotype control, it should match the primary antibody's host species and Ig class.
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Application WB ELISA IHC IP/ChIP ICC/IF FACS
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