M1 / M2 / TAM Marker Antibody Panel
货号 | 内含物名称 | 宿主克隆性 | 反应 | 应用 | 包装 |
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ARG10514 | anti-CD68 antibody [KP1] | Mouse mAb | Hu, Rat | FACS, ICC/IF, IHC-P, WB | 20 μg |
ARG56509 | anti-iNOS antibody | Rabbit pAb | Hu, Ms, Rat, Mamm | ICC/IF, IHC-Fr, IHC-P, IP, WB | 50 μl |
ARG55554 | anti-CD206 / MMR antibody [15-2] | Mouse mAb | Hu, Ms | CyTOF®-candidate, FACS, FuncSt, ICC/IF, IHC-Fr, IP, WB | 20 μg |
ARG66630 | anti-CD163 antibody [SQab19148] | Rabbit mAb | Hu | IHC-P | 20 μl |
产品描述 | M1 / M2 / TAM Marker Antibody Panel is an all-in-one solution to make identification of macrophage subtypes easy and economic. This antibody panel comprises M1 macrophage markers CD68 and iNOS antibodies, and M2 macrophage markers CD163 and CD206 antibodies. Besides, all four antibodies are ideal for determining the tumor-associated macrophage (TAM) phenotype. All the antibodies in this panel have excellent IHC staining performance. Also, the different host species make double staining possible. Related news: New antibody panels and duos for Tumor immune microenvironment Anti-SerpinB9 therapy, a new strategy for cancer therapy RIP1 activation and pathogenesis of NASH |
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靶点名称 | M1 / M2 / TAM Marker |
別名 | M1/M2/TAM Marker antibody; CD68 antibody; CD206 / MMR antibody; iNOS antibody; CD163 antibody |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
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注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
ARG56509 anti-iNOS antibody IHC-P image
Immunohistochemistry: Rat Brain stained with ARG56509 anti-iNOS antibody at 1:100 dilution.
From Abrar Roshdy Abouelkeir et al. European Chemical Bulletin,(2023) doi: 10.31838/ecb/2023.12.1.470, Fig. 6.
ARG56509 anti-iNOS antibody ICC/IF image (Customer's Feedback)
Immunofluorescence: RAW264.7 cells were fixed with 4% paraformaldehyde for 15 min at RT, permeabilized with 0.1% Triton X-100 then blocked with 2% albumin for 60 min at RT. Cells were stained with ARG56509 anti-iNOS antibody (green) at 4°C. DAPI (blue) was used as the nuclear counter stain.
ARG56509 anti-iNOS antibody WB image
Western blot: Rat Aortic stained with ARG56509 anti-iNOS antibody at 1:1000 dilution.
From Wahid Shah et al. Sci Rep. (2023), doi: 10.1038/s41598-023-43786-4, Fig. 2. C.
ARG10514 anti-CD68 antibody [KP1] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human tonsil stained with ARG10514 anti-CD68 antibody [KP1]. Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0).
ARG55554 anti-CD206 / MMR antibody [15-2] IHC-Fr image
Immunohistochemistry: Frozen section of Human tonsil tissue stained with ARG55554 anti-CD206 / MMR antibody [15-2] at 1:25 dilution. The antibody stains endothelia of lymph vessels strongly.
ARG56509 anti-iNOS antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human pancreatic ductal adenocarcinoma stained with ARG56509 anti-iNOS antibody.
ARG56509 anti-iNOS antibody WB image
Western blot: Raw264.7 cells untreated or treated with LPS. 20 µg of cell lysates stained with ARG56509 anti-iNOS antibody at 1:400 dilution.
ARG66630 anti-CD163 antibody [SQab19148] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human liver tissue stained with ARG66630 anti-CD163 antibody [SQab19148]. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).
ARG10514 anti-CD68 antibody [KP1] WB image
Western blot: Human Macrophages and N1 Fibroblast untreated or treated with N-glycosidase F. The blots were stained with ARG10514 anti-CD68 antibody [KP1].
ARG55554 anti-CD206 / MMR antibody [15-2] FACS image
Flow Cytometry: Stimulated (GM-CSF + IL-4) human peripheral blood mononuclear cells stained with ARG55554 anti-CD206 / MMR antibody [15-2] at 9 µg/ml dilution, followed by PE-conjugated Goat anti-Mouse antibody.
ARG10514 anti-CD68 antibody [KP1] FACS image
Flow Cytometry: THP1 cells prefixed with 4% PFA and then permeabilised with 0.25% saponin. Cells were stained with ARG10514 anti-CD68 antibody [KP1] (white area) or isotype control antibody (gray area).
ARG10514 anti-CD68 antibody [KP1] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast carcinoma stained with ARG10514 anti-CD68 antibody [KP1].
ARG55554 anti-CD206 / MMR antibody [15-2] FACS image
Flow Cytometry: Separation of human CD206 positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed). Human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained with ARG55554 anti-CD206 / MMR antibody [15-2] at 9 µg/ml dilution, followed by PE-conjugated Goat anti-Mouse antibody.