Mouse CA4 / Carbonic Anhydrase 4 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
- 标准范围:
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Brand:
CAT.NO. : ARG82425
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82425-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82425-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82425-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82425-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82425-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82425-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82425-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82425-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82425-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82425-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82425-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82425 Mouse CA4 / Carbonic Anhydrase 4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CA4 / Carbonic Anhydrase 4 in serum, plasma (EDTA, heparin, citrate), cell culture supernatants and urine. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 靶点名称 | CA4 / Carbonic Anhydrase 4 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 50 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin, citrate), cell culture supernatants and urine. |
| 标准范围 | 78 - 5000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.4% Inter-Assay CV: 5.9% |
| 別名 | CAIV; EC 4.2.1.1; Carbonic anhydrase IV; Car4; CA-IV; Carbonate dehydratase IV; RP17; Carbonic anhydrase 4 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | CA4 |
|---|---|
| 全名 | carbonic anhydrase IV |
| 背景介绍 | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Its exact function is not known; however, it may have a role in inherited renal abnormalities of bicarbonate transport. [provided by RefSeq, Jul 2008] |
| 生物功能 | Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid. [UniProt] |
| 细胞定位 | Cell membrane; Lipid-anchor, GPI-anchor. [UniProt] |
| 产品亮点 | Related products: Carbonic Anhydrase antibodies; Carbonic Anhydrase ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG82425 Mouse CA4 / Carbonic Anhydrase 4 ELISA Kit standard curve image
ARG82425 Mouse CA4 / Carbonic Anhydrase 4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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