Mouse CA9 / Carbonic Anhydrase 9 ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81976
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81976-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81976-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81976-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81976-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81976-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81976-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81976-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81976-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81976-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81976-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81976-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81976 Mouse CA9 / Carbonic Anhydrase 9 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CA9 / Carbonic Anhydrase 9 in serum, plasma (heparin, EDTA, citrate), urine and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | CA9 / Carbonic Anhydrase 9 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 7.8 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA, citrate), urine and cell culture supernatants. |
| 标准范围 | 15.6 - 1000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.8%; Inter-Assay CV: 7.6% |
| 別名 | EC 4.2.1.1; MN; CAIX; pMW1; Carbonic anhydrase 9; Carbonic anhydrase IX; RCC-associated antigen G250; Membrane antigen MN; P54/58N; Carbonate dehydratase IX; Renal cell carcinoma-associated antigen G250; CA-IX |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | CA9 |
|---|---|
| 全名 | carbonic anhydrase IX |
| 背景介绍 | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014] |
| 生物功能 | Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. [UniProt] |
| 产品亮点 | Related products: Carbonic Anhydrase antibodies; Carbonic Anhydrase ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Asn-346 bears high-mannose type glycan structures. [UniProt] |
检测图片 (1)
ARG81976 Mouse CA9 / Carbonic Anhydrase 9 ELISA Kit standard curve image
ARG81976 Mouse CA9 / Carbonic Anhydrase 9 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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